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dc.contributor.authorTanwar, Hanumant S
dc.contributor.authorKhoo, Keith K
dc.contributor.authorGarvey, Megan
dc.contributor.authorWaddington, Lynne
dc.contributor.authorLeis, Andrew
dc.contributor.authorHijnen, Marcel
dc.contributor.authorVelkov, Tony
dc.contributor.authorDumsday, Geoff J
dc.contributor.authorMcKinstry, William J
dc.contributor.authorMak, Johnson
dc.date.accessioned2023-06-15T23:33:40Z
dc.date.available2023-06-15T23:33:40Z
dc.date.issued2017
dc.identifier.issn1553-7366en_US
dc.identifier.doi10.1371/journal.ppat.1006221en_US
dc.identifier.urihttp://hdl.handle.net/10072/409519
dc.description.abstractThe interactions that occur during HIV Pr55Gagoligomerization and genomic RNA packaging are essential elements that facilitate HIV assembly. However, mechanistic details of these interactions are not clearly defined. Here, we overcome previous limitations in producing large quantities of full-length recombinant Pr55Gagthat is required for isothermal titration calorimetry (ITC) studies, and we have revealed the thermodynamic properties of HIV assembly for the first time. Thermodynamic analysis showed that the binding between RNA and HIV Pr55Gagis an energetically favourable reaction (ΔG<0) that is further enhanced by the oligomerization of Pr55Gag. The change in enthalpy (ΔH) widens sequentially from: (1) Pr55Gag-Psi RNA binding during HIV genome selection; to (2) Pr55Gag-Guanosine Uridine (GU)-containing RNA binding in cytoplasm/plasma membrane; and then to (3) Pr55Gag-Adenosine(A)-containing RNA binding in immature HIV. These data imply the stepwise increments of heat being released during HIV biogenesis may help to facilitate the process of viral assembly. By mimicking the interactions between A-containing RNA and oligomeric Pr55Gagin immature HIV, it was noted that a p6 domain truncated Pr50Gag Δp6is less efficient than full-length Pr55Gagin this thermodynamic process. These data suggest a potential unknown role of p6 in Pr55Gag-Pr55Gagoligomerization and/or Pr55Gag-RNA interaction during HIV assembly. Our data provide direct evidence on how nucleic acid sequences and the oligomeric state of Pr55Gagregulate HIV assembly.en_US
dc.description.peerreviewedYesen_US
dc.languageEnglishen_US
dc.publisherPUBLIC LIBRARY SCIENCEen_US
dc.relation.ispartofjournalPLOS Pathogensen_US
dc.relation.ispartofvolume13en_US
dc.subject.fieldofresearchMicrobiologyen_US
dc.subject.fieldofresearchImmunologyen_US
dc.subject.fieldofresearchMedical microbiologyen_US
dc.subject.fieldofresearchcode3107en_US
dc.subject.fieldofresearchcode3204en_US
dc.subject.fieldofresearchcode3207en_US
dc.subject.keywordsScience & Technologyen_US
dc.subject.keywordsLife Sciences & Biomedicineen_US
dc.subject.keywordsMicrobiologyen_US
dc.subject.keywordsParasitologyen_US
dc.subject.keywordsVirologyen_US
dc.titleThe thermodynamics of Pr55(Gag)-RNA interaction regulate the assembly of HIVen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Articlesen_US
dcterms.bibliographicCitationTanwar, HS; Khoo, KK; Garvey, M; Waddington, L; Leis, A; Hijnen, M; Velkov, T; Dumsday, GJ; McKinstry, WJ; Mak, J, The thermodynamics of Pr55(Gag)-RNA interaction regulate the assembly of HIV, PLOS Pathogens, 2017, 13en_US
dcterms.dateAccepted2017-02-06
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/en_US
dc.date.updated2021-10-28T04:13:10Z
dc.description.versionVersion of Record (VoR)en_US
gro.rights.copyright© 2017 Tanwar et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
gro.hasfulltextFull Text
gro.griffith.authorMak, Johnson


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