Inter-lamellar nanostructures-by-design for high-performance dual-photoelectrode photofuel cell based genosensor
Author(s)
Sun, Yuping
Li, Fen
Liu, Xiaoqiang
Qin, Tengteng
Li, Tongtong
Zheng, Hejie
Alwarappan, Subbiah
Ostrikov, Kostya Ken
Griffith University Author(s)
Year published
2022
Metadata
Show full item recordAbstract
Photofuel cells (PFCs) that integrate “clean-solar-to-electric energy” and “sensing platform” can enable next-generation biosensors for diverse health care and environmental applications. However, the inferior performance of photoelectrodes often results in insufficient solar-to-power conversion for reliable molecular detection. To overcome this problem, we developed a PFC-based genosensor by combining a Ti3C2Tx|CdS photoanode and a CuInS2 photocathode to generate significant output power for the reliable detection of miRNA-21. The innovative photoanode design involves (i) the insertion of CdS nanorods into multi-layered ...
View more >Photofuel cells (PFCs) that integrate “clean-solar-to-electric energy” and “sensing platform” can enable next-generation biosensors for diverse health care and environmental applications. However, the inferior performance of photoelectrodes often results in insufficient solar-to-power conversion for reliable molecular detection. To overcome this problem, we developed a PFC-based genosensor by combining a Ti3C2Tx|CdS photoanode and a CuInS2 photocathode to generate significant output power for the reliable detection of miRNA-21. The innovative photoanode design involves (i) the insertion of CdS nanorods into multi-layered Ti3C2Tx to expand the interlamellar space from ~0.98 to ~1.34 nm for accommodating more biomolecules to improve the sensor sensitivity; (ii) the formation of the nanoscale Schottky junction to enhance photo-generated charge separation and photoelectric conversion rates. The hybridization of capture DNA, assistant DNA and miRNA-21 on the photoanode led to the initial quenching of output signal of prepared PFC. As a mimic enzyme, manganese porphyrin was intercalated into the conjugated DNA double chains to catalyze a precipitation reaction on the photoanode surface, which resulted in a further signal quenching. Due to the combination of specially designed PFC with dual signal quenching, the proposed PFC-based genosensor exhibited a dynamic detection range of 1 fM to 0.01 μM with a LOD of 0.053 fM for detection of miRNA-21.
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View more >Photofuel cells (PFCs) that integrate “clean-solar-to-electric energy” and “sensing platform” can enable next-generation biosensors for diverse health care and environmental applications. However, the inferior performance of photoelectrodes often results in insufficient solar-to-power conversion for reliable molecular detection. To overcome this problem, we developed a PFC-based genosensor by combining a Ti3C2Tx|CdS photoanode and a CuInS2 photocathode to generate significant output power for the reliable detection of miRNA-21. The innovative photoanode design involves (i) the insertion of CdS nanorods into multi-layered Ti3C2Tx to expand the interlamellar space from ~0.98 to ~1.34 nm for accommodating more biomolecules to improve the sensor sensitivity; (ii) the formation of the nanoscale Schottky junction to enhance photo-generated charge separation and photoelectric conversion rates. The hybridization of capture DNA, assistant DNA and miRNA-21 on the photoanode led to the initial quenching of output signal of prepared PFC. As a mimic enzyme, manganese porphyrin was intercalated into the conjugated DNA double chains to catalyze a precipitation reaction on the photoanode surface, which resulted in a further signal quenching. Due to the combination of specially designed PFC with dual signal quenching, the proposed PFC-based genosensor exhibited a dynamic detection range of 1 fM to 0.01 μM with a LOD of 0.053 fM for detection of miRNA-21.
View less >
Journal Title
Sensors and Actuators B: Chemical
Volume
350
Subject
Analytical chemistry
Materials engineering
Atomic, molecular and optical physics
Science & Technology
Physical Sciences
Chemistry, Analytical
Electrochemistry