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dc.contributor.authorFoongladda, S
dc.contributor.authorBanu, S
dc.contributor.authorPholwat, S
dc.contributor.authorGratz, J
dc.contributor.authorO-Thong, S
dc.contributor.authorNakkerd, N
dc.contributor.authorChinli, R
dc.contributor.authorFerdous, SS
dc.contributor.authorRahman, SMM
dc.contributor.authorRahman, A
dc.contributor.authorAhmed, S
dc.contributor.authorHeysell, S
dc.contributor.authorSariko, M
dc.contributor.authorKibiki, G
dc.contributor.authorHoupt, E
dc.description.abstractBackground: Although phenotypic drug susceptibility testing (DST) is endorsed as the standard for secondline drug testing of Mycobacterium tuberculosis, it is slow and laborious. Methods: We evaluated the accuracy of two faster, easier methodologies that provide results for multiple drugs: a genotypic TaqMan® Array Card (TAC) and the Sensititrew MYCOTBTM plate. Both methods were tested at three central laboratories in Bangladesh, Tanzania, and Thailand with 212 multidrug-resistant tuberculosis (MDR-TB) isolates and compared with the laboratories' phenotypic method in use. Results: The overall accuracy for ethambutol, streptomycin, amikacin, kanamycin, ofloxacin, and moxifloxacin vs. the phenotypic standard was 87% for TAC (range 70-99) and 88% for the MYCOTB plate (range 76-98). To adjudicate discordances, we re-defined the standard as the consensus of the three methods, against which the TAC and MYCOTB plate yielded 94-95% accuracy, while the phenotypic result yielded 93%. Some isolates with genotypic mutations and high minimum inhibitory concentration (MIC) were phenotypically susceptible, and some isolates without mutations and low MIC were phenotypically resistant, questioning the phenotypic standard. Conclusions: In our view, the TAC, the MYCOTB plate, and the conventional phenotypic method have similar performance for second-line drugs; however, the former methods offer speed, throughput, and quantitative DST information.en_US
dc.publisherInternational Union Against Tuberculosis and Lung Diseaseen_US
dc.relation.ispartofjournalInternational Journal of Tuberculosis and Lung Diseaseen_US
dc.subject.fieldofresearchCardiovascular medicine and haematologyen_US
dc.titleComparison of TaqMan Array Card and MYCOTB with conventional phenotypic susceptibility testing in MDR-TBen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Articlesen_US
dcterms.bibliographicCitationFoongladda, S; Banu, S; Pholwat, S; Gratz, J; O-Thong, S; Nakkerd, N; Chinli, R; Ferdous, SS; Rahman, SMM; Rahman, A; Ahmed, S; Heysell, S; Sariko, M; Kibiki, G; Houpt, E, Comparison of TaqMan Array Card and MYCOTB with conventional phenotypic susceptibility testing in MDR-TB, International Journal of Tuberculosis and Lung Disease, 2016, 20 (8), pp. 1105-1112en_US
gro.hasfulltextNo Full Text
gro.griffith.authorRahman, Sabuktagin

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