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dc.contributor.authorHolmes, Roger S
dc.contributor.authorSpradling-Reeves, Kimberly D
dc.contributor.authorCox, Laura A
dc.date.accessioned2023-07-05T03:05:06Z
dc.date.available2023-07-05T03:05:06Z
dc.date.issued2017
dc.identifier.issn2153-0602en_US
dc.identifier.doi10.4172/2153-0602.1000211en_US
dc.identifier.urihttp://hdl.handle.net/10072/410081
dc.description.abstractGlutamyl aminopeptidase (ENPEP) is a member of the M1 family of endopeptidases which are mammalian type II integral membrane zinc-containing endopeptidases. ENPEP is involved in the catabolic pathway of the renin-angiotensin system forming angiotensin III, which participates in blood pressure regulation and blood vessel formation. Comparative ENPEP amino acid sequences and structures and ENPEP gene locations were examined using data from several mammalian genome projects. Mammalian ENPEP sequences shared 71-98% identities. Five N-glycosylation sites were conserved for all mammalian ENPEP proteins examined although 9-18 sites were observed, in each case. Sequence alignments, key amino acid residues and predicted secondary and tertiary structures were also studied, including transmembrane and cytoplasmic sequences and active site residues. Highest levels of human ENPEP expression were observed in the terminal ileum of the small intestine and in the kidney cortex. Mammalian ENPEP genes contained 20 coding exons. The human ENPEP gene promoter and first coding exon contained a CpG island (CpG27) and at least 6 transcription factor binding sites, whereas the 3'-UTR region contained 7 miRNA target sites, which may contribute to the regulation of ENPEP gene expression in tissues of the body. Phylogenetic analyses examined the relationships of mammalian ENPEP genes and proteins, including primate, other eutherian, marsupial and monotreme sources, using chicken ENPEP as a primordial sequence for comparative purposes.en_US
dc.description.peerreviewedYesen_US
dc.languageEnglishen_US
dc.publisherLongdom Publishingen_US
dc.publisher.urihttps://www.walshmedicalmedia.com/abstract/mammalian-glutamyl-aminopeptidase-genes-enpep-and-proteins-comparative-studies-of-a-major-contributor-to-arterial-hypert-16211.htmlen_US
dc.relation.ispartofpagefrom1000211en_US
dc.relation.ispartofissue2en_US
dc.relation.ispartofjournalJournal of Data Mining in Genomics & Proteomicsen_US
dc.relation.ispartofvolume8en_US
dc.subject.fieldofresearchBiomedical and clinical sciencesen_US
dc.subject.fieldofresearchcode32en_US
dc.subject.keywordsAmino acid sequenceen_US
dc.subject.keywordsAminopeptidase Aen_US
dc.subject.keywordsArterial hypertensionAbbreviations: ENPEP: Glutamyl Aminopeptidaseen_US
dc.subject.keywordsBLAST: Basic Local Alignment Search Toolen_US
dc.subject.keywordsBLAT: Blast-Like Alignment Toolen_US
dc.titleMammalian glutamyl aminopeptidase genes (ENPEP) and proteins: Comparative studies of a major contributor to arterial hypertensionen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Articlesen_US
dcterms.bibliographicCitationHolmes, RS; Spradling-Reeves, KD; Cox, LA, Mammalian glutamyl aminopeptidase genes (ENPEP) and proteins: Comparative studies of a major contributor to arterial hypertension, Journal of Data Mining in Genomics & Proteomics, 2017, 8 (2), pp. 1000211en_US
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/en_US
dc.date.updated2021-11-15T02:14:06Z
dc.description.versionVersion of Record (VoR)en_US
gro.rights.copyright© 2017 Holmes RS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
gro.hasfulltextFull Text
gro.griffith.authorHolmes, Roger S.


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