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dc.contributor.authorGonzaga, ZJC
dc.contributor.authorChen, S
dc.contributor.authorLehoux, M
dc.contributor.authorSegura, M
dc.contributor.authorRehm, BHA
dc.date.accessioned2021-12-15T08:17:41Z
dc.date.available2021-12-15T08:17:41Z
dc.date.issued2021
dc.identifier.issn2076-393X
dc.identifier.doi10.3390/vaccines9121386
dc.identifier.urihttp://hdl.handle.net/10072/410838
dc.description.abstractStreptococcus suis is a zoonotic pathogen affecting pigs and humans. This bacterium causes severe economic losses in the swine industry and poses a serious threat to public health and food safety. There is no effective commercial vaccine available for pigs or humans. In this study, we applied the biopolymer particle (BP) vaccine technology to incorporate seven conserved S. suis antigens (38 kDa protein (38), enolase (Enol), SSU1915, SSU1355, SSU0185, SSU1215, and SSU1773 (SSU1 and SSU2)). Two combinations of these antigens (38 and Enol; all SSU antigens designated as SSU1 and SSU2) were engineered to mediate production of BPs coated with either antigens 38 and Enol or SSU1 and SSU2 inside recombinant Escherichia coli. The isolated and purified empty BPs, 38-BP-Enol and SSU1-BP-SSU2, showed size ranges of 312–428 nm and 292–344 nm with and without the QuilA® adjuvant, respectively, and all showed a negative surface charge. Further characterization of purified BPs confirmed the presence of the expected antigen-comprising fusion proteins as assessed by tryptic peptide fingerprinting analysis using quadrupole time-of-flight mass spectrometry and im-munoblotting. Vaccination with 38-BP-Enol and SSU1-BP-SSU2 formulated with and without QuilA® adjuvant induced significant antigen-specific humoral immune responses in mice. Antigen-coated BPs induced significant and specific Ig (IgM + IgG) and IgG immune responses (1.0 × 106–1.0 × 107) when compared with mice vaccinated with empty BPs. Functionality of the immune response was confirmed in challenge experiments using an acute murine S. suis infection model, which showed 100% survival of the 38-BP-Enol and SSU1-BP-SSU2 vaccinated mice compared to 70% survival when vaccinated with empty BPs. Overall, our data suggest that S. suis antigen-coated BPs could be developed into particulate vaccines that induce protective immunity against S. suis infections.
dc.description.peerreviewedYes
dc.languageen
dc.publisherMDPI AG
dc.relation.ispartofpagefrom1386
dc.relation.ispartofissue12
dc.relation.ispartofjournalVaccines
dc.relation.ispartofvolume9
dc.subject.fieldofresearchBiomedical engineering
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchImmunology
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchcode4003
dc.subject.fieldofresearchcode3202
dc.subject.fieldofresearchcode3204
dc.subject.fieldofresearchcode3207
dc.titleEngineering antigens to assemble into polymer particle vaccines for prevention of streptococcus suis infection
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationGonzaga, ZJC; Chen, S; Lehoux, M; Segura, M; Rehm, BHA, Engineering antigens to assemble into polymer particle vaccines for prevention of streptococcus suis infection, Vaccines, 2021, 9 (12), pp. 1386
dcterms.licensehttps:// creativecommons.org/licenses/by/ 4.0/
dc.date.updated2021-12-08T22:29:14Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorChen, Shuxiong
gro.griffith.authorRehm, Bernd


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