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  • In vitro modulation of Schwann cell behavior by VEGF and PDGF in an inflammatory environment

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    Author(s)
    Basu, Souptik
    Choudhury, Indra N
    Nazareth, Lynn
    Chacko, Anu
    Shelper, Todd
    Vial, Marie-Laure
    Ekberg, Jenny AK
    St John, James A
    Griffith University Author(s)
    St John, James A.
    Ekberg, Jenny A.
    Basu, Souptik
    Vial, Marie-Laure
    Shelper, Todd B.
    Year published
    2022
    Metadata
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    Abstract
    Peripheral glial cell transplantation with Schwann cells (SCs) is a promising approach for treating spinal cord injury (SCI). However, improvements are needed and one avenue to enhance regenerative functional outcomes is to combine growth factors with cell transplantation. Vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) are neuroprotective, and a combination of these factors has improved outcomes in rat SCI models. Thus, transplantation of SCs combined with VEGF and PDGF may further improve regenerative outcomes. First, however, we must understand how the two factors modulate SCs. In this ...
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    Peripheral glial cell transplantation with Schwann cells (SCs) is a promising approach for treating spinal cord injury (SCI). However, improvements are needed and one avenue to enhance regenerative functional outcomes is to combine growth factors with cell transplantation. Vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) are neuroprotective, and a combination of these factors has improved outcomes in rat SCI models. Thus, transplantation of SCs combined with VEGF and PDGF may further improve regenerative outcomes. First, however, we must understand how the two factors modulate SCs. In this in vitro study, we show that an inflammatory environment decreased the rate of SC-mediated phagocytosis of myelin debris but the addition of VEGF and PDGF (alone and combined) improved phagocytosis. Cytokine expression by SCs in the inflammatory environment revealed that addition of PDGF led to significantly lower level of pro-inflammatory cytokine, TNF-α, but IL-6 and anti-inflammatory cytokines (TGF-β and IL-10), remained unaltered. Further, PDGF was able to decrease the expression of myelination associated gene Oct6 in the presence of inflammatory environment. Overall, these results suggest that the use of VEGF and/or PDGF combined with SC transplantation may be beneficial in SCI therapy.
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    Journal Title
    Scientific Reports
    Volume
    12
    Issue
    1
    DOI
    https://doi.org/10.1038/s41598-021-04222-7
    Copyright Statement
    © The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
    Subject
    Clinical sciences
    Publication URI
    http://hdl.handle.net/10072/411482
    Collection
    • Journal articles

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