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dc.contributor.authorLiyanage, Chamikara
dc.contributor.authorWathupola, Asanga
dc.contributor.authorMuraleetharan, Sanjayan
dc.contributor.authorPerera, Kanthi
dc.contributor.authorPunyadeera, Chamindie
dc.contributor.authorUdagama, Preethi
dc.date.accessioned2022-02-07T05:33:34Z
dc.date.available2022-02-07T05:33:34Z
dc.date.issued2019
dc.identifier.issn2218-273X
dc.identifier.doi10.3390/biom9040148
dc.identifier.urihttp://hdl.handle.net/10072/412078
dc.description.abstractSilencing of tumor-suppressor genes (TSGs) by DNA promoter hypermethylation is an early event in carcinogenesis; hence, TSGs may serve as early tumor biomarkers. We determined the promoter methylation levels of p16INK4a, RASSF1A, TIMP3, and PCQAP/MED15 TSGs in salivary DNA from oral cancer (OC) and oropharyngeal cancer (OPC) patients, using methylation-specific PCR coupled with densitometry analysis. We assessed the association between DNA methylation of individual TSGs with OC and OPC risk factors. The performance and the clinical validity of this quadruple-methylation marker panel were evaluated in discriminating OC and OPC patients from healthy controls using the CombiROC web tool. Our study reports that RASSF1A, TIMP3, and PCQAP/MED15 TSGs were significantly hypermethylated in OC and OPC cases compared to healthy controls. DNA methylation levels of TSGs were significantly augmented by smoking, alcohol use, and betel quid chewing, indicating the fact that frequent exposure to risk factors may drive oral and oropharyngeal carcinogenesis through TSG promoter hypermethylation. Also, this quadruple-methylation marker panel of p16INK4a, RASSF1A, TIMP3, and PCQAP/MED15 TSGs demonstrated excellent diagnostic accuracy in the early detection of OC at 91.7% sensitivity and 92.3% specificity and of OPC at 99.8% sensitivity and 92.1% specificity from healthy controls.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherMDPI
dc.relation.ispartofpagefrom148
dc.relation.ispartofissue4
dc.relation.ispartofjournalBiomolecules
dc.relation.ispartofvolume9
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchcode3101
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsBiochemistry & Molecular Biology
dc.subject.keywordsoral cancer
dc.subject.keywordsoropharyngeal cancer
dc.titlePromoter Hypermethylation of Tumor-Suppressor Genes p16(INK4a), RASSF1A, TIMP3, and PCQAP/MED15 in Salivary DNA as a Quadruple Biomarker Panel for Early Detection of Oral and Oropharyngeal Cancers
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationLiyanage, C; Wathupola, A; Muraleetharan, S; Perera, K; Punyadeera, C; Udagama, P, Promoter Hypermethylation of Tumor-Suppressor Genes p16(INK4a), RASSF1A, TIMP3, and PCQAP/MED15 in Salivary DNA as a Quadruple Biomarker Panel for Early Detection of Oral and Oropharyngeal Cancers, Biomolecules, 2019, 9 (4), pp. 148
dcterms.dateAccepted2019-04-08
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.date.updated2022-02-07T05:31:44Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorPunyadeera, Chamindie


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