Show simple item record

dc.contributor.authorKulasinghe, Arutha
dc.contributor.authorZhou, Jian
dc.contributor.authorKenny, Liz
dc.contributor.authorPapautsky, Ian
dc.contributor.authorPunyadeera, Chamindie
dc.date.accessioned2022-02-07T05:44:20Z
dc.date.available2022-02-07T05:44:20Z
dc.date.issued2019
dc.identifier.issn2072-6694
dc.identifier.doi10.3390/cancers11010089
dc.identifier.urihttp://hdl.handle.net/10072/412082
dc.description.abstractCirculating tumour cells (CTCs) are the metastatic precursors to distant disease in head and neck cancers (HNCs). Whilst the prognostic and predictive value of single CTCs have been well documented, the role of CTC clusters, which potentially have a higher metastatic capacity are limited. In this study, the authors used a novel straight microfluidic chip to focus and capture CTCs. The chip offers high cell recoveries with clinically relevant numbers (10-500 cells/mL) without the need for further purification. Single CTCs were identified in 10/21 patient samples (range 2-24 CTCs/mL), CTC clusters in 9/21 patient samples (range 1-6 CTC clusters/mL) and circulating tumour microemboli (CTM) in 2/21 samples. This study demonstrated that CTC clusters contain EGFR amplified single CTCs within the cluster volume. This novel microfluidic chip demonstrates the efficient sorting and preservation of single CTCs, CTC clusters and CTMs. The authors intend to expand this study to a larger cohort to determine the clinical implication of the CTC subsets in HNC.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherMDPI
dc.relation.ispartofpagefrom89
dc.relation.ispartofissue1
dc.relation.ispartofjournalCancers
dc.relation.ispartofvolume11
dc.subject.fieldofresearchOncology and carcinogenesis
dc.subject.fieldofresearchcode3211
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsOncology
dc.subject.keywordscirculating tumour cells (CTCs)
dc.subject.keywordsCTC clusters
dc.titleCapture of Circulating Tumour Cell Clusters Using Straight Microfluidic Chips
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationKulasinghe, A; Zhou, J; Kenny, L; Papautsky, I; Punyadeera, C, Capture of Circulating Tumour Cell Clusters Using Straight Microfluidic Chips, Cancers, 2019, 11 (1), pp. 89
dcterms.dateAccepted2019-01-08
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.date.updated2022-02-07T05:42:20Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorPunyadeera, Chamindie


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record