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dc.contributor.authorLim, Yenkai
dc.contributor.authorFukuma, Naoki
dc.contributor.authorTotsika, Makrina
dc.contributor.authorKenny, Liz
dc.contributor.authorMorrison, Mark
dc.contributor.authorPunyadeera, Chamindie
dc.date.accessioned2022-02-07T06:00:28Z
dc.date.available2022-02-07T06:00:28Z
dc.date.issued2018
dc.identifier.issn2235-2988
dc.identifier.doi10.3389/fcimb.2018.00267
dc.identifier.urihttp://hdl.handle.net/10072/412086
dc.description.abstractThe oral microbiome can play a role in the instigation and progression of oral diseases that can manifest into other systemic conditions. These associations encourage the exploration of oral dysbiosis leading to the pathogenesis of cancers. In this study, oral rinse was used to characterize the oral microbiome fluctuation associated with oral cavity cancer (OCC) and oropharyngeal cancers (OPC). The study cohort consists of normal healthy controls (n = 10, between 20 and 30 years of age; n = 10, above 50 years of age), high-risk individuals (n = 11, above 50 years of age with bad oral hygiene and/or oral diseases) and OCC and OPC patients (n = 31, HPV-positive; n = 21, HPV-negative). Oral rinse samples were analyzed using 16S rRNA gene amplicon sequencing on the MiSeq platform. Kruskal-Wallis rank test was used to identify genera associated with OCC and OPC. A logistic regression analysis was carried out to determine the performance of these genera as a biomarker panel to predict OCC and OPC. In addition, a two-fold cross-validation with a bootstrap procedure was carried out in R to investigate how well the panel would perform in an emulated clinical scenario. Our data indicate that the oral microbiome is able to predict the presence of OCC and OPC with sensitivity and specificity of 100 and 90%, respectively. With further validation, the panel could potentially be implemented into clinical diagnostic and prognostic workflows for OCC and OPC.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherFrontiers Media SA
dc.relation.ispartofpagefrom267
dc.relation.ispartofjournalFrontiers in Cellular and Infection Microbiology
dc.relation.ispartofvolume8
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3107
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsImmunology
dc.subject.keywordsMicrobiology
dc.subject.keywordsoral cancer
dc.titleThe Performance of an Oral Microbiome Biomarker Panel in Predicting Oral Cavity and Oropharyngeal Cancers
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationLim, Y; Fukuma, N; Totsika, M; Kenny, L; Morrison, M; Punyadeera, C, The Performance of an Oral Microbiome Biomarker Panel in Predicting Oral Cavity and Oropharyngeal Cancers, Frontiers in Cellular and Infection Microbiology, 2018, 8, pp. 267
dcterms.dateAccepted2018-07-17
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.date.updated2022-02-07T05:57:20Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2018 Lim, Fukuma, Totsika, Kenny, Morrison and Punyadeera. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
gro.hasfulltextFull Text
gro.griffith.authorPunyadeera, Chamindie


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