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  • Within-Day Baseline Variation in Salivary Biomarkers in Healthy Men

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    Punyadeera521799-Accepted.pdf (332.5Kb)
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    Accepted Manuscript (AM)
    Author(s)
    Idris, Firman Prathama
    Wan, Yunxia
    Zhang, Xi
    Punyadeera, Chamindie
    Griffith University Author(s)
    Punyadeera, Chamindie
    Year published
    2017
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    Abstract
    Saliva is an easily accessible sample and offers practical and noninvasive biomarker solutions as an alternative to blood and urine-based diagnostics. Saliva contains a plethora of biomolecules such as nucleic acids, hormones, proteins, and electrolytes. On the other hand, little is known on the extent to which the biomolecules in saliva vary over time within a given person. This baseline information is crucial for future development of robust saliva-based diagnostics. We have collected unstimulated whole mouth saliva from 20 healthy young men at four times during the day, including before and after a meal. We measured the ...
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    Saliva is an easily accessible sample and offers practical and noninvasive biomarker solutions as an alternative to blood and urine-based diagnostics. Saliva contains a plethora of biomolecules such as nucleic acids, hormones, proteins, and electrolytes. On the other hand, little is known on the extent to which the biomolecules in saliva vary over time within a given person. This baseline information is crucial for future development of robust saliva-based diagnostics. We have collected unstimulated whole mouth saliva from 20 healthy young men at four times during the day, including before and after a meal. We measured the salivary cortisol, testosterone, C-reactive protein (CRP), stability of genomic DNA (gDNA) and DNA methylation levels of APC, P16INK4a, and PCQAP in these samples. We found that the salivary CRP, DNA methylation, and CD44 gDNA levels did not vary significantly across four time points (p > 0.05) while the salivary cortisol and testosterone levels significantly varied from the morning collection to the afternoon collection (p < 0.05). Furthermore, salivary cortisol levels were significantly affected by eating (p < 0.05). Our study offers a within-person baseline temporal assessment of several clinically relevant biomolecules and diagnostics, and suggests that salivary cortisol and testosterone levels vary over time in a given day whereas CRP and DNA methylation of tumor suppressor genes and CD44 amplification are stable throughout the day. Future research and clinical applications of salivary biomarkers and diagnostics should take into consideration their temporal variations.
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    Journal Title
    OMICS: A Journal of Integrative Biology
    Volume
    21
    Issue
    2
    DOI
    https://doi.org/10.1089/omi.2016.0168
    Copyright Statement
    This is a copy of an article published in the OMICS: A Journal of Integrative Biology. Copyright 2017 Mary Ann Liebert, Inc. OMICS: A Journal of Integrative Biology is available online at: http://www.liebertonline.com
    Subject
    Biological sciences
    Science & Technology
    Life Sciences & Biomedicine
    Biotechnology & Applied Microbiology
    Genetics & Heredity
    biomarkers
    Publication URI
    http://hdl.handle.net/10072/412099
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    • Journal articles

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