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  • Analysis of the extreme diversity of salivary alpha-amylase isoforms generated by physiological proteolysis using liquid chromatography-tandem mass spectrometry

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    Punyadeera521867-Accepted.pdf (818.4Kb)
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    Author(s)
    Bailey, Ulla-Maja
    Punyadeera, Chamindie
    Cooper-White, Justin J
    Schulz, Benjamin L
    Griffith University Author(s)
    Punyadeera, Chamindie
    Year published
    2012
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    Abstract
    Saliva is a crucial biofluid for oral health and is also of increasing importance as a non-invasive source of disease biomarkers. Salivary alpha-amylase is an abundant protein in saliva, and changes in amylase expression have been previously associated with a variety of diseases and conditions. Salivary alpha-amylase is subject to a high diversity of post-translational modifications, including physiological proteolysis in the oral cavity. Here we developed methodology for rapid sample preparation and non-targeted LC-ESI-MS/MS analysis of saliva from healthy subjects and observed an extreme diversity of alpha-amylase proteolytic ...
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    Saliva is a crucial biofluid for oral health and is also of increasing importance as a non-invasive source of disease biomarkers. Salivary alpha-amylase is an abundant protein in saliva, and changes in amylase expression have been previously associated with a variety of diseases and conditions. Salivary alpha-amylase is subject to a high diversity of post-translational modifications, including physiological proteolysis in the oral cavity. Here we developed methodology for rapid sample preparation and non-targeted LC-ESI-MS/MS analysis of saliva from healthy subjects and observed an extreme diversity of alpha-amylase proteolytic isoforms. Our results emphasize the importance of consideration of post-translational events such as proteolysis in proteomic studies, biomarker discovery and validation, particularly in saliva. © 2012 Elsevier B.V.
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    Journal Title
    Journal of Chromatography B
    Volume
    911
    DOI
    https://doi.org/10.1016/j.jchromb.2012.10.023
    Copyright Statement
    © 2012 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
    Subject
    Science & Technology
    Life Sciences & Biomedicine
    Physical Sciences
    Biochemical Research Methods
    Chemistry, Analytical
    Publication URI
    http://hdl.handle.net/10072/412159
    Collection
    • Journal articles

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