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  • Three-Dimensional Flow-Through Protein Platform

    Author(s)
    van Lieshout, RML
    van Domburg, T
    Saalmink, M
    Verbeek, R
    Wimberger-Friedl, R
    van Dieijen-Visser, MP
    Punyadeera, C
    Griffith University Author(s)
    Punyadeera, Chamindie
    Year published
    2009
    Metadata
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    Abstract
    We have developed a new protein microarray (Immuno-Flow Protein Platform, IFPP) that utilizes a porous nitrocellulose (NC) membrane with printed spots of capture probes. The sample is pumped actively through the NC membrane, to enhance binding efficiency and introduce stringency. Compared to protein microarrays assayed with the conventional incubation-shaking method the rate of binding is enhanced on the IFPP by at least a factor of 10, so that the total assay time can be reduced drastically without compromising sensitivity. Similarly, the sensitivity can be improved.We demonstrate the detection of 1 pM of C-reactive protein ...
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    We have developed a new protein microarray (Immuno-Flow Protein Platform, IFPP) that utilizes a porous nitrocellulose (NC) membrane with printed spots of capture probes. The sample is pumped actively through the NC membrane, to enhance binding efficiency and introduce stringency. Compared to protein microarrays assayed with the conventional incubation-shaking method the rate of binding is enhanced on the IFPP by at least a factor of 10, so that the total assay time can be reduced drastically without compromising sensitivity. Similarly, the sensitivity can be improved.We demonstrate the detection of 1 pM of C-reactive protein (CRP) in 70 μL of plasma within a total assay time of 7 min. The small sample and reagent volumes, combined with the speed of the assay, make our IFPP also well-suited for a point-of-care/near-patient setting. The potential clinical application of the IFPP is demonstrated by validating CRP detection both in human plasma and serum samples against standard clinical laboratory methods. © 2009 American Chemical Society.
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    Journal Title
    Analytical Chemistry
    Volume
    81
    Issue
    13
    DOI
    https://doi.org/10.1021/ac801244d
    Subject
    Science & Technology
    Physical Sciences
    Chemistry, Analytical
    Chemistry
    C-REACTIVE PROTEIN
    Publication URI
    http://hdl.handle.net/10072/412200
    Collection
    • Journal articles

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