dc.contributor.author | Mohamed, Raafat | |
dc.contributor.author | Shajimoon, Aravindra | |
dc.contributor.author | Afroz, Rizwana | |
dc.contributor.author | Gabr, Mai | |
dc.contributor.author | Thomas, Walter G | |
dc.contributor.author | Little, Peter J | |
dc.contributor.author | Kamato, Danielle | |
dc.date.accessioned | 2022-02-18T00:52:41Z | |
dc.date.available | 2022-02-18T00:52:41Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 1742-464X | |
dc.identifier.doi | 10.1111/febs.16297 | |
dc.identifier.uri | http://hdl.handle.net/10072/412450 | |
dc.description.abstract | Transforming growth factor (TGF)-β signalling commences with the engagement of TGF-β ligand to cell surface TGF-β receptors (TGFBR) stimulating Smad2 carboxyl-terminal phosphorylation (phospho-Smad2C) and downstream biological responses. In several cell models, G protein-coupled receptors (GPCRs) transactivate the TGF-β receptors type-1 (TGFBR1) leading to phospho-Smad2C, however, we have recently published that in keratinocytes thrombin did not transactivate the TGFBR1. The bulk of TGFBRs reside in the cytosol and in response to protein kinase B (Akt phosphorylation) can translocate to the cell surface increasing the cell's responsiveness to TGF-β. In this study, we investigate the role of Akt in GPCR transactivation of the TGFBR1. We demonstrate that angiotensin II and thrombin do not phosphorylate Smad2C in human vascular smooth muscle cells and in keratinocytes respectively. We used Akt agonist, SC79 to sensitise the cells to Akt and observed that Ang II and thrombin phosphorylate Smad2C via Akt/AS160-dependent pathways. We show that SC79 rapidly translocates TGFBRs to the cell surface thus increasing the cell's response to the GPCR agonist. These findings highlight novel mechanistic insight for the role of Akt in GPCR transactivation of the TGFBR1.
k | |
dc.description.peerreviewed | Yes | |
dc.language | en | |
dc.publisher | Wiley | |
dc.relation.ispartofjournal | The FEBS Journal | |
dc.subject.fieldofresearch | Biochemistry and cell biology | |
dc.subject.fieldofresearch | Medical biochemistry and metabolomics | |
dc.subject.fieldofresearch | Medicinal and biomolecular chemistry | |
dc.subject.fieldofresearchcode | 3101 | |
dc.subject.fieldofresearchcode | 3205 | |
dc.subject.fieldofresearchcode | 3404 | |
dc.title | Akt acts as a switch for GPCR transactivation of the TGF‐β receptor type 1 | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dcterms.bibliographicCitation | Mohamed, R; Shajimoon, A; Afroz, R; Gabr, M; Thomas, WG; Little, PJ; Kamato, D, Akt acts as a switch for GPCR transactivation of the TGF‐β receptor type 1, The FEBS Journal | |
dc.date.updated | 2022-02-17T05:15:42Z | |
gro.description.notepublic | This publication has been entered as an advanced online version in Griffith Research Online. | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Afroz, Rizwana | |
gro.griffith.author | Kamato, Danielle | |