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  • The impact of acute lung injury, ECMO and transfusion on oxidative stress and plasma selenium levels in an ovine model

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    Fung158741-Accepted.pdf (321.4Kb)
    File version
    Accepted Manuscript (AM)
    Author(s)
    McDonald, Charles I
    Fung, Yoke Lin
    Shekar, Kiran
    Diab, Sara D
    Dunster, Kimble R
    Passmore, Margaret R
    Foley, Samuel R
    Simonova, Gabriela
    Platts, David
    Fraser, John F
    Griffith University Author(s)
    Fung, Lin
    Fraser, John F.
    Year published
    2015
    Metadata
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    Abstract
    The purpose of this study was to determine the effects of smoke induced acute lung injury (S-ALI), extracorporeal membrane oxygenation (ECMO) and transfusion on oxidative stress and plasma selenium levels. Forty ewes were divided into (i) healthy control (. n=. 4), (ii) S-ALI control (. n=. 7), (iii) ECMO control (. n=. 7), (iv) S-ALI. +. ECMO (. n=. 8) and (v) S-ALI. +. ECMO. +. packed red blood cell (PRBC) transfusion (. n=. 14). Plasma thiobarbituric acid reactive substances (TBARS), selenium and glutathione peroxidase (GPx) activity were analysed at baseline, after smoke injury (or sham) and 0.25, 1, 2, 6, 7, 12 and 24. ...
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    The purpose of this study was to determine the effects of smoke induced acute lung injury (S-ALI), extracorporeal membrane oxygenation (ECMO) and transfusion on oxidative stress and plasma selenium levels. Forty ewes were divided into (i) healthy control (. n=. 4), (ii) S-ALI control (. n=. 7), (iii) ECMO control (. n=. 7), (iv) S-ALI. +. ECMO (. n=. 8) and (v) S-ALI. +. ECMO. +. packed red blood cell (PRBC) transfusion (. n=. 14). Plasma thiobarbituric acid reactive substances (TBARS), selenium and glutathione peroxidase (GPx) activity were analysed at baseline, after smoke injury (or sham) and 0.25, 1, 2, 6, 7, 12 and 24. h after initiation of ECMO. Peak TBARS levels were similar across all groups. Plasma selenium decreased by 54% in S-ALI sheep (1.36. ±. 0.20 to 0.63. ±. 0.27. μmol/L, p<. 0.0001), and 72% in sheep with S-ALI. +. ECMO at 24. h (1.36. ±. 0.20 to 0.38. ±. 0.19, p<. 0.0001). PRBC transfusion had no effect on TBARS, selenium levels or glutathione peroxidase activity in plasma. While ECMO independently increased TBARS in healthy sheep to levels which were similar to the S-ALI control, the addition of ECMO after S-ALI caused a negligible increase in TBARS. This suggests that the initial lung injury was the predominant feature in the TBARS response. In contrast, the addition of ECMO in S-ALI sheep exacerbated reductions in plasma selenium beyond that of S-ALI or ECMO alone. Clinical studies are needed to confirm the extent and duration of selenium loss associated with ECMO.
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    Journal Title
    Journal of Trace Elements in Medicine and Biology
    Volume
    30
    DOI
    https://doi.org/10.1016/j.jtemb.2015.01.004
    Copyright Statement
    © 2015 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
    Subject
    Biochemistry and cell biology
    Science & Technology
    Life Sciences & Biomedicine
    Biochemistry & Molecular Biology
    Endocrinology & Metabolism
    Extracorporeal membrane oxygenation
    Publication URI
    http://hdl.handle.net/10072/412686
    Collection
    • Journal articles

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