The impact of acute lung injury, ECMO and transfusion on oxidative stress and plasma selenium levels in an ovine model

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Author(s)
McDonald, Charles I
Fung, Yoke Lin
Shekar, Kiran
Diab, Sara D
Dunster, Kimble R
Passmore, Margaret R
Foley, Samuel R
Simonova, Gabriela
Platts, David
Fraser, John F
Year published
2015
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The purpose of this study was to determine the effects of smoke induced acute lung injury (S-ALI), extracorporeal membrane oxygenation (ECMO) and transfusion on oxidative stress and plasma selenium levels. Forty ewes were divided into (i) healthy control (. n=. 4), (ii) S-ALI control (. n=. 7), (iii) ECMO control (. n=. 7), (iv) S-ALI. +. ECMO (. n=. 8) and (v) S-ALI. +. ECMO. +. packed red blood cell (PRBC) transfusion (. n=. 14). Plasma thiobarbituric acid reactive substances (TBARS), selenium and glutathione peroxidase (GPx) activity were analysed at baseline, after smoke injury (or sham) and 0.25, 1, 2, 6, 7, 12 and 24. ...
View more >The purpose of this study was to determine the effects of smoke induced acute lung injury (S-ALI), extracorporeal membrane oxygenation (ECMO) and transfusion on oxidative stress and plasma selenium levels. Forty ewes were divided into (i) healthy control (. n=. 4), (ii) S-ALI control (. n=. 7), (iii) ECMO control (. n=. 7), (iv) S-ALI. +. ECMO (. n=. 8) and (v) S-ALI. +. ECMO. +. packed red blood cell (PRBC) transfusion (. n=. 14). Plasma thiobarbituric acid reactive substances (TBARS), selenium and glutathione peroxidase (GPx) activity were analysed at baseline, after smoke injury (or sham) and 0.25, 1, 2, 6, 7, 12 and 24. h after initiation of ECMO. Peak TBARS levels were similar across all groups. Plasma selenium decreased by 54% in S-ALI sheep (1.36. ±. 0.20 to 0.63. ±. 0.27. μmol/L, p<. 0.0001), and 72% in sheep with S-ALI. +. ECMO at 24. h (1.36. ±. 0.20 to 0.38. ±. 0.19, p<. 0.0001). PRBC transfusion had no effect on TBARS, selenium levels or glutathione peroxidase activity in plasma. While ECMO independently increased TBARS in healthy sheep to levels which were similar to the S-ALI control, the addition of ECMO after S-ALI caused a negligible increase in TBARS. This suggests that the initial lung injury was the predominant feature in the TBARS response. In contrast, the addition of ECMO in S-ALI sheep exacerbated reductions in plasma selenium beyond that of S-ALI or ECMO alone. Clinical studies are needed to confirm the extent and duration of selenium loss associated with ECMO.
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View more >The purpose of this study was to determine the effects of smoke induced acute lung injury (S-ALI), extracorporeal membrane oxygenation (ECMO) and transfusion on oxidative stress and plasma selenium levels. Forty ewes were divided into (i) healthy control (. n=. 4), (ii) S-ALI control (. n=. 7), (iii) ECMO control (. n=. 7), (iv) S-ALI. +. ECMO (. n=. 8) and (v) S-ALI. +. ECMO. +. packed red blood cell (PRBC) transfusion (. n=. 14). Plasma thiobarbituric acid reactive substances (TBARS), selenium and glutathione peroxidase (GPx) activity were analysed at baseline, after smoke injury (or sham) and 0.25, 1, 2, 6, 7, 12 and 24. h after initiation of ECMO. Peak TBARS levels were similar across all groups. Plasma selenium decreased by 54% in S-ALI sheep (1.36. ±. 0.20 to 0.63. ±. 0.27. μmol/L, p<. 0.0001), and 72% in sheep with S-ALI. +. ECMO at 24. h (1.36. ±. 0.20 to 0.38. ±. 0.19, p<. 0.0001). PRBC transfusion had no effect on TBARS, selenium levels or glutathione peroxidase activity in plasma. While ECMO independently increased TBARS in healthy sheep to levels which were similar to the S-ALI control, the addition of ECMO after S-ALI caused a negligible increase in TBARS. This suggests that the initial lung injury was the predominant feature in the TBARS response. In contrast, the addition of ECMO in S-ALI sheep exacerbated reductions in plasma selenium beyond that of S-ALI or ECMO alone. Clinical studies are needed to confirm the extent and duration of selenium loss associated with ECMO.
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Journal Title
Journal of Trace Elements in Medicine and Biology
Volume
30
Copyright Statement
© 2015 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
Subject
Biochemistry and cell biology
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Endocrinology & Metabolism
Extracorporeal membrane oxygenation