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  • Calcium Contributes to Polarized Targeting of HIV Assembly Machinery by Regulating Complex Stability

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    Kishor841344-Published.pdf (7.213Mb)
    Author(s)
    Kishor, Chandan
    Spillings, Belinda L
    Luhur, Johana
    Lutomski, Corinne A
    Lin, Chi-Hung
    McKinstry, William J
    Day, Christopher J
    Jennings, Michael P
    Jarrold, Martin F
    Mak, Johnson
    Griffith University Author(s)
    Mak, Johnson
    Jennings, Michael P.
    de Villiers, Belinda
    Luhur, Johana T.
    Day, Christopher J.
    Kishor, Chandan
    Lin, Chi-Hung
    Year published
    2022
    Metadata
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    Abstract
    Polarized or precision targeting of protein complexes to their destinations is fundamental to cellular homeostasis, but the mechanism underpinning directional protein delivery is poorly understood. Here, we use the uropod targeting HIV synapse as a model system to show that the viral assembly machinery Gag is copolarized with the intracellular calcium (Ca2+) gradient and binds specifically with Ca2+. Conserved glutamic/aspartic acids flanking endosomal sorting complexes required for transport binding motifs are major Ca2+ binding sites. Deletion or mutation of these Ca2+ binding residues resulted in altered protein trafficking ...
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    Polarized or precision targeting of protein complexes to their destinations is fundamental to cellular homeostasis, but the mechanism underpinning directional protein delivery is poorly understood. Here, we use the uropod targeting HIV synapse as a model system to show that the viral assembly machinery Gag is copolarized with the intracellular calcium (Ca2+) gradient and binds specifically with Ca2+. Conserved glutamic/aspartic acids flanking endosomal sorting complexes required for transport binding motifs are major Ca2+ binding sites. Deletion or mutation of these Ca2+ binding residues resulted in altered protein trafficking phenotypes, including (i) changes in the Ca2+-Gag distribution relationship during uropod targeting and/or (ii) defects in homo/hetero-oligomerization with Gag. Mutation of Ca2+ binding amino acids is associated with enhanced ubiquitination and a decline in virion release via uropod protein complex delivery. Our data that show Ca2+-protein binding, via the intracellular Ca2+ gradient, represents a mechanism that regulates intracellular protein trafficking.
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    Journal Title
    JACS Au
    Volume
    2
    Issue
    2
    DOI
    https://doi.org/10.1021/jacsau.1c00563
    Copyright Statement
    © 2022 The Authors. Published by American Chemical Society. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International (CC BY-NC-ND 4.0) License, which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
    Subject
    Virology
    Publication URI
    http://hdl.handle.net/10072/413130
    Collection
    • Journal articles

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