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dc.contributor.authorBarisam, Maryam
dc.contributor.authorNiavol, Fazeleh Ranjbar
dc.contributor.authorKinj, Moslem Afrasiabi
dc.contributor.authorSaidi, Mohammad Said
dc.contributor.authorGhanbarian, Hossein
dc.contributor.authorKashaninejad, Navid
dc.date.accessioned2022-04-11T04:00:47Z
dc.date.available2022-04-11T04:00:47Z
dc.date.issued2022
dc.identifier.issn2468-2179
dc.identifier.doi10.1016/j.jsamd.2022.100439
dc.identifier.urihttp://hdl.handle.net/10072/413819
dc.description.abstractCurrent chemotherapies can often kill fast-growing cancer cells but are ineffective in destroying cancer stem cells (CSCs). This study aimed to investigate the effect of in vitro cell culture conditions on the features of lung adenocarcinoma cells (represented by A549, non-small cell type (NSCLC), cell lines) and the induction of cancer stem-like cells. For this purpose, a microfluidic system containing U-shaped arrays was designed and numerically optimized. This system was used for the three-dimensional culture of cells under a continuous laminar flow of culture medium. Numerical simulations were also performed to estimate the quiescent and necrotic zones inside the spheroids and the fluid shear stress on the cultured spheroids. Moreover, the effects of culture medium flow rate, oxygen and glucose concentrations and anoikis phenomenon on the number of CD90+ cells and expression of stemness, epithelial-mesenchymal transition (EMT) and ATP-binding cassette (ABC)-transporters genes were investigated. The results showed that all these parameters substantially affected the enrichment of A549 cancer stem-like cells. We also investigated the effect of the CSC enrichment method, i.e., shear stress and oxygen and glucose concentrations, on resistance to cisplatin treatment. Increasing shear stress and glucose concentration and decreasing oxygen concentration led to a sharp increase in chemoresistance of cells. Interestingly, changing oxygen concentration was more significant than changing the other factors. The results of this paper can be helpful for the effective enrichment of CSCs by adjusting in vitro culture conditions.
dc.description.peerreviewedYes
dc.languageen
dc.publisherElsevier BV
dc.relation.ispartofpagefrom100439
dc.relation.ispartofissue2
dc.relation.ispartofjournalJournal of Science: Advanced Materials and Devices
dc.relation.ispartofvolume7
dc.subject.fieldofresearchOncology and carcinogenesis
dc.subject.fieldofresearchcode3211
dc.titleEnrichment of cancer stem-like cells by controlling oxygen, glucose and fluid shear stress in a microfluidic spheroid culture device
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationBarisam, M; Niavol, FR; Kinj, MA; Saidi, MS; Ghanbarian, H; Kashaninejad, N, Enrichment of cancer stem-like cells by controlling oxygen, glucose and fluid shear stress in a microfluidic spheroid culture device, Journal of Science: Advanced Materials and Devices, 2022, 7 (2), pp. 100439
dcterms.licensehttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.date.updated2022-04-08T01:44:50Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2022 Vietnam National University, Hanoi. Published by Elsevier B.V. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International (CC BY-NC-ND 4.0) License, which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorKashaninejad, Navid


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