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  • Optimized Protocol for Protein Extraction from the Breast Tissue that is Compatible with Two-Dimensional Gel Electrophoresis

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    Author(s)
    Zakharchenko, Olena
    Greenwood, Christina
    Alldridge, Louise
    Souchelnytskyi, Serhiy
    Griffith University Author(s)
    Alldridge, Louise C.
    Year published
    2011
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    Abstract
    Proteomics is a highly informative approach to analyze cancer-associated transformation in tissues. The main challenge to use a tissue for proteomics studies is the small sample size and difficulties to extract and preserve proteins. The choice of a buffer compatible with proteomics applications is also a challenge. Here we describe a protocol optimized for the most efficient extraction of proteins from the human breast tissue in a buffer compatible with two-dimensional gel electrophoresis (2D-GE). This protocol is based on mechanically assisted disintegration of tissues directly in the 2D-GE buffer. Our method is simple, ...
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    Proteomics is a highly informative approach to analyze cancer-associated transformation in tissues. The main challenge to use a tissue for proteomics studies is the small sample size and difficulties to extract and preserve proteins. The choice of a buffer compatible with proteomics applications is also a challenge. Here we describe a protocol optimized for the most efficient extraction of proteins from the human breast tissue in a buffer compatible with two-dimensional gel electrophoresis (2D-GE). This protocol is based on mechanically assisted disintegration of tissues directly in the 2D-GE buffer. Our method is simple, robust and easy to apply in clinical practice. We demonstrate high quality of separation of proteins prepared according to the reported here protocol.
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    Journal Title
    Breast Cancer
    Volume
    18
    Issue
    5
    DOI
    https://doi.org/10.4137/BCBCR.S6263
    Copyright Statement
    © The Author(s) 2011. The attached file is reproduced here in accordance with the copyright policy of the publisher. For information about this journal please refer to the journal's website or contact the author.
    Subject
    Proteomics and Intermolecular Interactions (excl. Medical Proteomics)
    Oncology and Carcinogenesis
    Publication URI
    http://hdl.handle.net/10072/41383
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    • Journal articles

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