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dc.contributor.authorZakharchenko, Olena
dc.contributor.authorGreenwood, Christina
dc.contributor.authorAlldridge, Louise
dc.contributor.authorSouchelnytskyi, Serhiy
dc.date.accessioned2017-05-03T15:27:58Z
dc.date.available2017-05-03T15:27:58Z
dc.date.issued2011
dc.date.modified2011-11-07T06:55:33Z
dc.identifier.issn13406868
dc.identifier.doi10.4137/BCBCR.S6263
dc.identifier.urihttp://hdl.handle.net/10072/41383
dc.description.abstractProteomics is a highly informative approach to analyze cancer-associated transformation in tissues. The main challenge to use a tissue for proteomics studies is the small sample size and difficulties to extract and preserve proteins. The choice of a buffer compatible with proteomics applications is also a challenge. Here we describe a protocol optimized for the most efficient extraction of proteins from the human breast tissue in a buffer compatible with two-dimensional gel electrophoresis (2D-GE). This protocol is based on mechanically assisted disintegration of tissues directly in the 2D-GE buffer. Our method is simple, robust and easy to apply in clinical practice. We demonstrate high quality of separation of proteins prepared according to the reported here protocol.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent1599879 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer Japan KK
dc.publisher.placeJapan
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom37
dc.relation.ispartofpageto42
dc.relation.ispartofissue5
dc.relation.ispartofjournalBreast Cancer
dc.relation.ispartofvolume18
dc.rights.retentionY
dc.subject.fieldofresearchProteomics and Intermolecular Interactions (excl. Medical Proteomics)
dc.subject.fieldofresearchOncology and Carcinogenesis
dc.subject.fieldofresearchcode060109
dc.subject.fieldofresearchcode1112
dc.titleOptimized Protocol for Protein Extraction from the Breast Tissue that is Compatible with Two-Dimensional Gel Electrophoresis
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© The Author(s) 2011. The attached file is reproduced here in accordance with the copyright policy of the publisher. For information about this journal please refer to the journal's website or contact the author.
gro.date.issued2011
gro.hasfulltextFull Text
gro.griffith.authorAlldridge, Louise C.


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