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dc.contributor.authorSharmin, S
dc.contributor.authorZohura, FT
dc.contributor.authorIslam, MS
dc.contributor.authorShimonty, A
dc.contributor.authorKhan, MAAK
dc.contributor.authorParveen, R
dc.contributor.authorSharmin, F
dc.contributor.authorAhsan, CR
dc.contributor.authorIslam, ABMMK
dc.contributor.authorYasmin, M
dc.date.accessioned2022-05-03T02:30:11Z
dc.date.available2022-05-03T02:30:11Z
dc.date.issued2021
dc.identifier.issn1471-2407
dc.identifier.doi10.1186/s12885-021-07906-5
dc.identifier.urihttp://hdl.handle.net/10072/414260
dc.description.abstractBackground: Cervical cancer is a gynecologic cancer type that develops in the cervix, accounting for 8% mortality of all female cancer patients. Infection with specific human papillomavirus (HPV) types is considered the most severe risk factor for cervical cancer. In the context of our socioeconomic conditions, an increasing burden of this disease and high mortality rate prevail in Bangladesh. Although several researches related to the epidemiology, HPV vaccination, and treatment modalities were conducted, researches on the mutation profiles of marker genes in cervical cancer in Bangladesh remain unexplored. Methods: In this study, five different genomic regions within the top three most frequently mutated genes (EGFR, KRAS and PIK3CA) in COSMIC database with a key role in the development of cervical cancers were selected to study the mutation frequency in Bangladeshi patients. In silico analysis was done in two steps: nucleotide sequence analysis and its corresponding amino acid analysis. Results: DNA from 46 cervical cancer tissue samples were extracted and amplified by PCR, using 1 set of primers designed for EGFR and 2 sets of primers designed for two different regions of both PIK3CA and KRAS gene. In total, 39 mutations were found in 26 patient samples. Eleven different mutations (23.91%), twenty-four different mutations (52.17%) and four mutations (8.7%) were found in amplified EGFR, PIK3CA and KRAS gene fragments, respectively; among which 1 (EGFR) was common in seven patient samples and 2 (PIKCA) were found in more than 1 patient. Our study shows that except for KRAS, the frequency of observed mutations in our patients is higher than those reported earlier in other parts of the world. Most of the exonic mutations were found only in the PIK3CA and EGFR genes. Conclusions: The study can be used as a basis to build a mutation database for cervical cancer in Bangladesh with the possibility of targetable oncogenic mutations. Further explorations are needed to establish future diagnostics, personalized medicine decisions, and other pharmaceutical applications for specific cancer subtypes.
dc.description.peerreviewedYes
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.relation.ispartofpagefrom289
dc.relation.ispartofjournalBMC Cancer
dc.relation.ispartofvolume21
dc.subject.fieldofresearchOncology and carcinogenesis
dc.subject.fieldofresearchObstetrics and gynaecology
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchcode3211
dc.subject.fieldofresearchcode321502
dc.subject.fieldofresearchcode3202
dc.subject.keywordsCervical cancer
dc.subject.keywordsEGFR
dc.subject.keywordsHPV
dc.subject.keywordsKRAS
dc.subject.keywordsMutation
dc.subject.keywordsPIK3CA
dc.titleMutational profiles of marker genes of cervical carcinoma in Bangladeshi patients
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationSharmin, S; Zohura, FT; Islam, MS; Shimonty, A; Khan, MAAK; Parveen, R; Sharmin, F; Ahsan, CR; Islam, ABMMK; Yasmin, M, Mutational profiles of marker genes of cervical carcinoma in Bangladeshi patients, BMC Cancer, 2021, 21, pp. 289
dcterms.dateAccepted2021-02-12
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2022-04-28T00:46:39Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
gro.hasfulltextFull Text
gro.griffith.authorIslam, Md Sajedul


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