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dc.contributor.authorChehrehasa, Fatemeh
dc.contributor.authorEkberg, Jenny
dc.contributor.authorLineburg, K.
dc.contributor.authorAmaya, Daniel
dc.contributor.authorMackay-Sim, Alan
dc.contributor.authorSt John, James
dc.date.accessioned2017-05-03T15:26:56Z
dc.date.available2017-05-03T15:26:56Z
dc.date.issued2011
dc.date.modified2014-06-05T03:14:13Z
dc.identifier.urihttp://hdl.handle.net/10072/41694
dc.description.abstractOlfactory ensheathing cells (OECs) support the regeneration of olfactory sensory neurons throughout life. However, it remains unclear how OECs respond to a major injury and whether stem cells give rise to new OECs in those conditions. Purpose: To identify where OECs proliferate in the olfactory pathway and the regions to whic they migrate. Methods: We examined the proliferation and migration of OECs by surgically removing an olfactory bulb from neonatal mice. The outer layer of the olfactory bulb, the nerve fibre layer, is rich with OECs and thus bulbectomy removed the OECs of the olfactory bulb. The peripheral region of the olfactory nerve within the nasal cavity and the olfactory epithelium where the stem cells are located remained untouched. Proliferating cells were labelled by the thymidine analogue, ethynyl deoxyuridine (EdU) at different days after surgery (days 0-14) and animals (n=3 at each time point) were harvested either 4 hr later or up to 14 days later. Results: In the unilateral bulbectomy model, there was a large stimulation of OEC proliferation throughout the olfactory nerve up to 14 days after bulbectomy. Tracking cells that had proliferated revealed that stem cells lining the basal layer of the olfactory epithelium also gave rise to OECs that subsequently migrated along the length of the olfactory nerve. Conclusion: These results demonstrate that OECs actively respond to widespread degeneration of olfactory axons and that both local proliferation of OECs as well as stem cells give rise to new OECs that migrate along the olfactory nerve to the regions of need.
dc.description.publicationstatusYes
dc.format.extent234990 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherAustralian Neuroscience Society
dc.publisher.urihttp://www.ans.org.au/ans-annual-conference/
dc.publisher.urihttp://www.sallyjayconferences.com.au/ans2011/
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofconferencename31st Annual Meeting of the Australian Neuroscience Society (ANS 2011)
dc.relation.ispartofconferencetitleProceedings of the Australian Neuroscience Society
dc.relation.ispartofdatefrom2011-01-31
dc.relation.ispartofdateto2011-02-03
dc.relation.ispartoflocationAuckland, NZ
dc.rights.retentionY
dc.subject.fieldofresearchCellular Nervous System
dc.subject.fieldofresearchPeripheral Nervous System
dc.subject.fieldofresearchcode110902
dc.subject.fieldofresearchcode110905
dc.titleOlfactory ensheathing cells proliferate from stem sells after injury
dc.typeConference output
dc.type.descriptionE3 - Conferences (Extract Paper)
dc.type.codeE - Conference Publications
gro.rights.copyright© The Author(s) 2011. The attached file is reproduced here in accordance with the copyright policy of the publisher. For information about this conference please refer to the conference’s website or contact the authors.
gro.date.issued2011
gro.hasfulltextFull Text
gro.griffith.authorMackay-Sim, Alan
gro.griffith.authorAmaya, Daniel A.
gro.griffith.authorChehrehasa, Fatemeh
gro.griffith.authorSt John, James A.
gro.griffith.authorEkberg, Jenny A.


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