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dc.contributor.authorL. Mendz, Georgeen_US
dc.contributor.authorMegraud, Francisen_US
dc.contributor.authorKorolik, Victoriaen_US
dc.date.accessioned2017-05-03T11:42:38Z
dc.date.available2017-05-03T11:42:38Z
dc.date.issued2005en_US
dc.date.modified2010-08-20T06:25:10Z
dc.identifier.issn03028933en_US
dc.identifier.doi10.1007/s00203-004-0752-7en_AU
dc.identifier.urihttp://hdl.handle.net/10072/4182
dc.description.abstractThe catabolism of phosphonates (Phn) by Campylobacter spp. was investigated employing nuclear magnetic resonance spectroscopy and cell culture techniques. The bacteria were capable of cleaving the Phn bonds of different compounds, including -aminomethylphosphonate, phosphonoacetate and phenylphosphonate (PhePhn). The kinetic parameters of these activities were determined in vivo in intact cells and in situ in whole-cell lysates. Cleavage of Phn-bearing compounds was associated with the cell-wall and cytosolic fractions. Results from substrate competition experiments suggested that at least two enzyme activities appeared to be involved in the cleavage of carbon-phosphate (C-P) bonds. In silico analyses indicated that no genes orthologous to those encoding C-P bond-cleaving enzymes in other bacteria were present in the Campylobacter jejuni genome. In most bacteria studied, Phn catabolism is induced under conditions of phosphate limitation; however, in Campylobacter spp. these activities were expressed in cells grown in media rich in phosphate. In chemically defined media, PhePhn supported bacterial growth and proliferation at concentrations above 100 M in the absence of phosphate. Thus, Phn utilisation may be a survival mechanism of Campylobacter spp. in milieux lacking sufficient phosphate. The expression of these enzyme activities in media abundant in phosphate suggested also that they may have other physiological roles.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherSpringer-Verlagen_US
dc.publisher.placeGermanyen_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom113en_US
dc.relation.ispartofpageto120en_US
dc.relation.ispartofjournalArchives of Microbiologyen_US
dc.relation.ispartofvolume183en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchcode270301en_US
dc.titlePhosphonate Catabolism by Campylobacter spp.en_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2005
gro.hasfulltextNo Full Text


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