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dc.contributor.authorKraschnefski, Marken_US
dc.contributor.authorScott, Stacyen_US
dc.contributor.authorHolloway, Gavanen_US
dc.contributor.authorS. Coulson, Barbaraen_US
dc.contributor.authorvon Itzstein, Marken_US
dc.contributor.authorBlanchard, Helenen_US
dc.contributor.editorH. M. Einspahr and J. M. Gussen_US
dc.date.accessioned2017-05-03T11:05:41Z
dc.date.available2017-05-03T11:05:41Z
dc.date.issued2005en_US
dc.identifier.issn1744-3091en_US
dc.identifier.doi10.1107/S1744309105032999en_US
dc.identifier.urihttp://hdl.handle.net/10072/4189
dc.description.abstractRotaviruses exhibit host-specificity and the first crystallographic information on a rotavirus strain that infects humans is reported here. Recognition and attachment to host cells, leading to invasion and infection, is critically linked to the function of the outer capsid spike protein of the rotavirus particle. In some strains the VP8* component of the spike protein is implicated in recognition and binding of sialic-acid-containing cell-surface carbohydrates, thereby enabling infection by the virus. The cloning, expression, purification, crystallization and initial X-ray diffraction analysis of the VP8* core from human Wa rotavirus is reported. Two crystal forms (trigonal P3221 and monoclinic P21) have been obtained and X-ray diffraction data have been collected, enabling determination of the VP8*64-223 structure by molecular replacement.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.languageEnglishen_US
dc.language.isoen_US
dc.publisherBlackwell Publishingen_US
dc.publisher.placeOxford, Englanden_US
dc.relation.ispartofstudentpublicationYen_US
dc.relation.ispartofpagefrom989en_US
dc.relation.ispartofpageto993en_US
dc.relation.ispartofjournalActa Crystallographica Section F: Structural Biology and Crystallization Communicationsen_US
dc.relation.ispartofvolumeF61en_US
dc.rights.retentionYen_US
dc.subject.fieldofresearchcode250302en_US
dc.titleCloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the VP8* carbohydrate-binding protein of the human rotavirus strain Wa.en_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2015-01-30T00:43:16Z
gro.hasfulltextNo Full Text


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