• myGriffith
    • Staff portal
    • Contact Us⌄
      • Future student enquiries 1800 677 728
      • Current student enquiries 1800 154 055
      • International enquiries +61 7 3735 6425
      • General enquiries 07 3735 7111
      • Online enquiries
      • Staff phonebook
    View Item 
    •   Home
    • Griffith Research Online
    • Conference outputs
    • View Item
    • Home
    • Griffith Research Online
    • Conference outputs
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

  • All of Griffith Research Online
    • Communities & Collections
    • Authors
    • By Issue Date
    • Titles
  • This Collection
    • Authors
    • By Issue Date
    • Titles
  • Statistics

  • Most Popular Items
  • Statistics by Country
  • Most Popular Authors
  • Support

  • Contact us
  • FAQs
  • Admin login

  • Login
  • Analysis of a Novel JK-1 Gene Expression in Benign and Malignant Colorectal Tumors

    Author(s)
    Kasem, K
    Lam, A
    Griffith University Author(s)
    Smith, Robert A.
    Lam, Alfred K.
    Ariana, Armin S.
    Gerhardy, Ben
    Gopalan, Vinod
    Kasem, Kais Y.
    Year published
    2010
    Metadata
    Show full item record
    Abstract
    Introduction: Colorectal tumors are amongst the most common neoplasms affecting individuals in industrialized nations. A newly discovered gene, JK-1 (FAM134B) located in chromosome 5 p15.2 plays an important role in molecular pathogenesis of esophageal cancer. This study aims at examining the role of this gene in colorectal tumors by analyzing the differences in gene mRNA expression in both benign and malignant colorectal tumors in comparison with non-neoplastic samples of colorectal tissue. Methods: The study was performed on 162 colorectal adenocarcinoma, 32 benign adenoma and 20 non-neoplastic colorectal tissues. mRNA ...
    View more >
    Introduction: Colorectal tumors are amongst the most common neoplasms affecting individuals in industrialized nations. A newly discovered gene, JK-1 (FAM134B) located in chromosome 5 p15.2 plays an important role in molecular pathogenesis of esophageal cancer. This study aims at examining the role of this gene in colorectal tumors by analyzing the differences in gene mRNA expression in both benign and malignant colorectal tumors in comparison with non-neoplastic samples of colorectal tissue. Methods: The study was performed on 162 colorectal adenocarcinoma, 32 benign adenoma and 20 non-neoplastic colorectal tissues. mRNA was extracted from the prospectively collected tissues and converted into cDNA. Primers were designed for the JK-1 gene and gene expression levels were determined using real-time PCR. The expression levels were calculated as a ratio of the Ct value for JK-1 to GAPDH (housekeeping gene) and expressed as inverse ratio to reflect the changes in gene expression. Results: The JK1 mRNA was detected in all colorectal tissues. The mean inverse ratio for JK-1 gene in adenoma samples was 0.929 ᠰ.005 which was significantly higher than in non-tumor samples which was 0.901 ᠰ.009 (p=0.005). On the other hand, cancer samples had a mean inverse ratio of 0.875 ᠰ.006 which was significantly lower than in non-tumor tissues (p<0.001). Conclusions: The results of this study indicate that JK-1 expression has a role in the carcinogenesis in colorectal cancer. The under-expression of the gene in cancer suggests that loss of gene expression may promote tumor progression, that it may act as a tumor suppressor gene or that it relates to certain genetic events in tumor pathogenesis. However, over-expression of JK-1 in adenoma samples may indicate that JK-1 has a functional role in dysplastic benign colon tumors, a function that is disrupted once the tumor progresses to a more invasive form.
    View less >
    Conference Title
    VIRCHOWS ARCHIV
    Volume
    457
    Issue
    2
    Publisher URI
    http://www.genecure.ca/en/programs/canadian-human-genetics-conference/about-the-event/
    Subject
    Cancer Genetics
    Clinical Sciences
    Publication URI
    http://hdl.handle.net/10072/41966
    Collection
    • Conference outputs

    Footer

    Disclaimer

    • Privacy policy
    • Copyright matters
    • CRICOS Provider - 00233E

    Tagline

    • Gold Coast
    • Logan
    • Brisbane - Queensland, Australia
    First Peoples of Australia
    • Aboriginal
    • Torres Strait Islander