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dc.contributor.convenorGuy Rouleau
dc.contributor.authorKasem, K
dc.contributor.authorLam, A
dc.date.accessioned2017-05-03T15:35:13Z
dc.date.available2017-05-03T15:35:13Z
dc.date.issued2010
dc.date.modified2012-02-10T01:15:03Z
dc.identifier.issn0945-6317
dc.identifier.urihttp://hdl.handle.net/10072/41966
dc.description.abstractIntroduction: Colorectal tumors are amongst the most common neoplasms affecting individuals in industrialized nations. A newly discovered gene, JK-1 (FAM134B) located in chromosome 5 p15.2 plays an important role in molecular pathogenesis of esophageal cancer. This study aims at examining the role of this gene in colorectal tumors by analyzing the differences in gene mRNA expression in both benign and malignant colorectal tumors in comparison with non-neoplastic samples of colorectal tissue. Methods: The study was performed on 162 colorectal adenocarcinoma, 32 benign adenoma and 20 non-neoplastic colorectal tissues. mRNA was extracted from the prospectively collected tissues and converted into cDNA. Primers were designed for the JK-1 gene and gene expression levels were determined using real-time PCR. The expression levels were calculated as a ratio of the Ct value for JK-1 to GAPDH (housekeeping gene) and expressed as inverse ratio to reflect the changes in gene expression. Results: The JK1 mRNA was detected in all colorectal tissues. The mean inverse ratio for JK-1 gene in adenoma samples was 0.929 ᠰ.005 which was significantly higher than in non-tumor samples which was 0.901 ᠰ.009 (p=0.005). On the other hand, cancer samples had a mean inverse ratio of 0.875 ᠰ.006 which was significantly lower than in non-tumor tissues (p<0.001). Conclusions: The results of this study indicate that JK-1 expression has a role in the carcinogenesis in colorectal cancer. The under-expression of the gene in cancer suggests that loss of gene expression may promote tumor progression, that it may act as a tumor suppressor gene or that it relates to certain genetic events in tumor pathogenesis. However, over-expression of JK-1 in adenoma samples may indicate that JK-1 has a functional role in dysplastic benign colon tumors, a function that is disrupted once the tumor progresses to a more invasive form.
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherCanadian Gene Cure Foundation (CGCF)
dc.publisher.urihttp://www.genecure.ca/en/programs/canadian-human-genetics-conference/about-the-event/
dc.relation.ispartofstudentpublicationY
dc.relation.ispartofconferencename3rd Annual Canadian Human Genetics Conference
dc.relation.ispartofconferencetitleVIRCHOWS ARCHIV
dc.relation.ispartofdatefrom2010-04-18
dc.relation.ispartofdateto2010-04-21
dc.relation.ispartoflocationQuebec - Canada
dc.relation.ispartofpagefrom165
dc.relation.ispartofpagefrom2 pages
dc.relation.ispartofpageto166
dc.relation.ispartofpageto2 pages
dc.relation.ispartofissue2
dc.relation.ispartofvolume457
dc.rights.retentionY
dc.subject.fieldofresearchCancer Genetics
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchcode111203
dc.subject.fieldofresearchcode1103
dc.titleAnalysis of a Novel JK-1 Gene Expression in Benign and Malignant Colorectal Tumors
dc.typeConference output
dc.type.descriptionE3 - Conferences (Extract Paper)
dc.type.codeE - Conference Publications
gro.facultyGriffith Health, School of Medicine
gro.date.issued2010
gro.hasfulltextNo Full Text
gro.griffith.authorSmith, Robert A.
gro.griffith.authorLam, Alfred K.
gro.griffith.authorAriana, Armin S.
gro.griffith.authorGerhardy, Ben
gro.griffith.authorGopalan, Vinod
gro.griffith.authorKasem, Kais Y.


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    Contains papers delivered by Griffith authors at national and international conferences.

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