Chapter 8 - Temporal trends in metabolite profiles correspond with seasonal patterns of temperature and rainfall during field-scale ecotoxicology assessment
Author(s)
Melvin, Steven
Lanctot, Chantal
Doriean, nicholas
Bennett, William
Carroll, Anthony
Griffith University Author(s)
Year published
2022
Metadata
Show full item recordAbstract
The multiparameter nature of metabolomics data offers sensitivity paired with a potential to unravel the mechanistic basis of toxicological stress. However, sensitive multiparameter analyses may be susceptible to confounding factors in complex natural systems and carefully designed research is needed to identify and characterize metabolomic responses of target species to specific environmental variables. This case study explored seasonal variability in the metabolome of mosquitofish (Gambusia holbrooki) from two sites in Australia, a reference wetland, and a metal(loid)-contaminated wetland. Numerous metabolites were elevated ...
View more >The multiparameter nature of metabolomics data offers sensitivity paired with a potential to unravel the mechanistic basis of toxicological stress. However, sensitive multiparameter analyses may be susceptible to confounding factors in complex natural systems and carefully designed research is needed to identify and characterize metabolomic responses of target species to specific environmental variables. This case study explored seasonal variability in the metabolome of mosquitofish (Gambusia holbrooki) from two sites in Australia, a reference wetland, and a metal(loid)-contaminated wetland. Numerous metabolites were elevated in fish from the contaminated wetland, but with notable decreases in several metabolites involved with metal(loid) binding, detoxification, and excretion. Results were qualitatively similar between sampling events, but seasonal differences in meteorological conditions and water quality corresponded with quantitative differences in the metabolome of fish. Metabolomics data representative of a wide range of environmental and site characteristics is critical to fully characterize chemically mediated effects between sampling locations. Similar research exploring other factors that may influence the metabolome of aquatic organism in complex environments (e.g., time of day, seasonal food availability, tidal cycles) is warranted.
View less >
View more >The multiparameter nature of metabolomics data offers sensitivity paired with a potential to unravel the mechanistic basis of toxicological stress. However, sensitive multiparameter analyses may be susceptible to confounding factors in complex natural systems and carefully designed research is needed to identify and characterize metabolomic responses of target species to specific environmental variables. This case study explored seasonal variability in the metabolome of mosquitofish (Gambusia holbrooki) from two sites in Australia, a reference wetland, and a metal(loid)-contaminated wetland. Numerous metabolites were elevated in fish from the contaminated wetland, but with notable decreases in several metabolites involved with metal(loid) binding, detoxification, and excretion. Results were qualitatively similar between sampling events, but seasonal differences in meteorological conditions and water quality corresponded with quantitative differences in the metabolome of fish. Metabolomics data representative of a wide range of environmental and site characteristics is critical to fully characterize chemically mediated effects between sampling locations. Similar research exploring other factors that may influence the metabolome of aquatic organism in complex environments (e.g., time of day, seasonal food availability, tidal cycles) is warranted.
View less >
Book Title
Applied Environmental Metabolomics: Community Insights and Guidance from the Field
Subject
Biochemistry and cell biology
Pollution and contamination