• myGriffith
    • Staff portal
    • Contact Us⌄
      • Future student enquiries 1800 677 728
      • Current student enquiries 1800 154 055
      • International enquiries +61 7 3735 6425
      • General enquiries 07 3735 7111
      • Online enquiries
      • Staff phonebook
    View Item 
    •   Home
    • Griffith Research Online
    • Conference outputs
    • View Item
    • Home
    • Griffith Research Online
    • Conference outputs
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

  • All of Griffith Research Online
    • Communities & Collections
    • Authors
    • By Issue Date
    • Titles
  • This Collection
    • Authors
    • By Issue Date
    • Titles
  • Statistics

  • Most Popular Items
  • Statistics by Country
  • Most Popular Authors
  • Support

  • Contact us
  • FAQs
  • Admin login

  • Login
  • The motility of olfactory ensheathing cells: a hitchhiker's guide to olfactory axon migration

    Author(s)
    Windus, Louisa
    Claxton, C.
    Mackay-Sim, Alan
    Key, B.
    St John, James
    Griffith University Author(s)
    Mackay-Sim, Alan
    Windus, Louisa
    St John, James A.
    Year published
    2010
    Metadata
    Show full item record
    Abstract
    During development and during regeneration in the adult, olfactory ensheathing cells (OECs) are intimately associated with axons of olfactory sensory neurons along their entire trajectory. It is assumed that OECs are essential for axon growth but little is known about how OECs and axons interact and respond to each other. We hypothesised that the motility of OECs directs the migration of olfactory axons. To address this we used timelapse imaging of fluorescently labelled primary cultures of olfactory sensory neurons and OECs to determine the mechanisms of olfactory axon extension and OEC interactions. We reveal here for the ...
    View more >
    During development and during regeneration in the adult, olfactory ensheathing cells (OECs) are intimately associated with axons of olfactory sensory neurons along their entire trajectory. It is assumed that OECs are essential for axon growth but little is known about how OECs and axons interact and respond to each other. We hypothesised that the motility of OECs directs the migration of olfactory axons. To address this we used timelapse imaging of fluorescently labelled primary cultures of olfactory sensory neurons and OECs to determine the mechanisms of olfactory axon extension and OEC interactions. We reveal here for the first time that the extension of pioneer olfactory axons is largely dependent on the motility of the underlying OECs. This intimate association is in part initiated and mediated by lamellipodial waves along the shaft of OEC processes. Moreover, as axons remain adhered to axons at all times, perturbation of OEC movement via GDNF and inhibitors of the JNK and SRC kinases significantly altered axon motility (n=20). We also reveal that inhibition of NCAM significantly disrupted OEC cell-cell recognition (n=10) resulting in increased OEC migration, while OEC-axon adhesion was maintained. Laser ablation of OECs, or substitution of OECs by Schwann cells, inhibited axon outgrowth. These results demonstrate that olfactory sensory axon outgrowth is dependent on cell-cell contact with OECs. Rather than merely providing support for axon growth the glia of the olfactory system strongly regulate the migration of olfactory axons.
    View less >
    Conference Title
    Proceedings of the Australian Society for Neuroscience Annual Meeting
    Publisher URI
    https://www.ans.org.au/resources/past-ans-conferences/57-past-ans-conferences
    Subject
    Peripheral Nervous System
    Central Nervous System
    Publication URI
    http://hdl.handle.net/10072/42220
    Collection
    • Conference outputs

    Footer

    Disclaimer

    • Privacy policy
    • Copyright matters
    • CRICOS Provider - 00233E
    • TEQSA: PRV12076

    Tagline

    • Gold Coast
    • Logan
    • Brisbane - Queensland, Australia
    First Peoples of Australia
    • Aboriginal
    • Torres Strait Islander