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dc.contributor.authorHolmes, Roger S
dc.contributor.authorCox, Laura A
dc.date.accessioned2017-05-03T14:56:15Z
dc.date.available2017-05-03T14:56:15Z
dc.date.issued2012
dc.date.modified2014-08-28T05:04:19Z
dc.identifier.issn2190-572X
dc.identifier.doi10.1007/s13205-011-0026-4
dc.identifier.urihttp://hdl.handle.net/10072/42457
dc.description.abstractGlycosylphosphatidylinositol-anchored high-density lipoprotein-binding protein 1 (GPIHBP1) functions as a platform and transport agent for lipoprotein lipase (LPL) which functions in the hydrolysis of chylomicrons, principally in heart, skeletal muscle and adipose tissue capillary endothelial cells. Previous reports of genetic deficiency for this protein have described severe chylomicronemia. Comparative GPIHBP1 amino acid sequences and structures and GPIHBP1 gene locations were examined using data from several mammalian genome projects. Mammalian GPIHBP1 genes usually contain four coding exons on the positive strand. Mammalian GPIHBP1 sequences shared 41-96% identities as compared with 9-32% sequence identities with other LY6-domain-containing human proteins (LY6-like). The human N-glycosylation site was predominantly conserved among other mammalian GPIHBP1 proteins except cow, dog and pig. Sequence alignments, key amino acid residues and conserved predicted secondary structures were also examined, including the N-terminal signal peptide, the acidic amino acid sequence region which binds LPL, the glycosylphosphatidylinositol linkage group, the Ly6 domain and the C-terminal a-helix. Comparative and phylogenetic studies of mammalian GPIHBP1 suggested that it originated in eutherian mammals from a gene duplication event of an ancestral LY6-like gene and subsequent integration of exon 2, which may have been derived from BCL11A (B-cell CLL/lymphoma 11A gene) encoding an extended acidic amino acid sequence.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent3264280 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringerOpen
dc.publisher.placeGermany
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom37
dc.relation.ispartofpageto52
dc.relation.ispartofissue1
dc.relation.ispartofjournal3 Biotech
dc.relation.ispartofvolume2
dc.rights.retentionY
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchGenetics not elsewhere classified
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode310599
dc.titleComparative studies of glycosylphosphatidylinositol-anchored high-density lipoprotein-binding protein 1: evidence for a eutherian mammalian origin for the GPIHBP1 gene from an LY6-like gene
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© The Author(s) 2011. This is a SpringerOpen Access license agreement which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.date.issued2012
gro.hasfulltextFull Text
gro.griffith.authorHolmes, Roger S.


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