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  • Stimulation of olfactory ensheathing cell motility enhances olfactory axon growth

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    Author(s)
    Windus, Louisa CE
    Chehrehasa, Fatemeh
    Lineburg, Katie E
    Claxton, Christina
    Mackay-Sim, Alan
    Key, Brian
    St John, James A
    Griffith University Author(s)
    Mackay-Sim, Alan
    St John, James A.
    Ekberg, Jenny A.
    Year published
    2011
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    Abstract
    Axons of primary olfactory neurons are intimately associated with olfactory ensheathing cells (OECs) from the olfactory epithelium until the final targeting of axons within the olfactory bulb. However, little is understood about the nature and role of interactions between OECs and axons during development of the olfactory nerve pathway. We have used high resolution time-lapse microscopy to examine the growth and interactions of olfactory axons and OECs in vitro. Transgenic mice expressing fluorescent reporters in primary olfactory axons (OMP-ZsGreen) and ensheathing cells (S100߭DsRed) enabled us to selectively analyse these ...
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    Axons of primary olfactory neurons are intimately associated with olfactory ensheathing cells (OECs) from the olfactory epithelium until the final targeting of axons within the olfactory bulb. However, little is understood about the nature and role of interactions between OECs and axons during development of the olfactory nerve pathway. We have used high resolution time-lapse microscopy to examine the growth and interactions of olfactory axons and OECs in vitro. Transgenic mice expressing fluorescent reporters in primary olfactory axons (OMP-ZsGreen) and ensheathing cells (S100߭DsRed) enabled us to selectively analyse these cell types in explants of olfactory epithelium. We reveal here that rather than providing only a permissive substrate for axon growth, OECs play an active role in modulating the growth of pioneer olfactory axons. We show that the interactions between OECs and axons were dependent on lamellipodial waves on the shaft of OEC processes. The motility of OECs was mediated by GDNF, which stimulated cell migration and increased the apparent motility of the axons, whereas loss of OECs via laser ablation of the cells inhibited olfactory axon outgrowth. These results demonstrate that the migration of OECs strongly regulates the motility of axons and that stimulation of OEC motility enhances axon extension and growth cone activity.
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    Journal Title
    Cellular and Molecular Life Sciences
    Volume
    68
    Issue
    19
    DOI
    https://doi.org/10.1007/s00018-011-0630-9
    Copyright Statement
    © 2011 Birkhäuser Basel. This is an electronic version of an article published in Cellular and Molecular Life Sciences, Vol. 68(19), pp. 3233-3247, 2011. Cellular and Molecular Life Sciences is available online at: http://www.springerlink.com/ with the open URL of your article.
    Subject
    Biochemistry and cell biology
    Zoology
    Clinical sciences
    Central nervous system
    Peripheral nervous system
    Medical biochemistry and metabolomics
    Oncology and carcinogenesis
    Publication URI
    http://hdl.handle.net/10072/42698
    Collection
    • Journal articles

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