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dc.contributor.authorGrage, Stephan L
dc.contributor.authorKeleshian, Asbed M
dc.contributor.authorTurdzeladze, Tamta
dc.contributor.authorBattle, Andrew R
dc.contributor.authorTay, Wee C
dc.contributor.authorMay, Roland P
dc.contributor.authorHolt, Stephen A
dc.contributor.authorContera, Sonia Antoranz
dc.contributor.authorHaertlein, Michael
dc.contributor.authorMoulin, Martine
dc.contributor.authorPal, Prithwish
dc.contributor.authorRohde, Paul R
dc.contributor.authorForsyth, V Trevor
dc.contributor.authorWatts, Anthony
dc.contributor.authorHuang, Kerwyn Casey
dc.contributor.authorUlrich, Anne S
dc.contributor.authorMartinac, Boris
dc.date.accessioned2017-05-03T16:04:28Z
dc.date.available2017-05-03T16:04:28Z
dc.date.issued2011
dc.date.modified2012-02-17T04:59:22Z
dc.identifier.issn0006-3495
dc.identifier.doi10.1016/j.bpj.2011.01.023
dc.identifier.urihttp://hdl.handle.net/10072/42845
dc.description.abstractMechanosensitive channels allow bacteria to respond to osmotic stress by opening a nanometer-sized pore in the cellular membrane. Although the underlying mechanism has been thoroughly studied on the basis of individual channels, the behavior of channel ensembles has yet to be elucidated. This work reveals that mechanosensitive channels of large conductance (MscL) exhibit a tendency to spatially cluster, and demonstrates the functional relevance of clustering. We evaluated the spatial distribution of channels in a lipid bilayer using patch-clamp electrophysiology, fluorescence and atomic force microscopy, and neutron scattering and reflection techniques, coupled with mathematical modeling of the mechanics of a membrane crowded with proteins. The results indicate that MscL forms clusters under a wide range of conditions. MscL is closely packed within each cluster but is still active and mechanosensitive. However, the channel activity is modulated by the presence of neighboring proteins, indicating membrane-mediated protein-protein interactions. Collectively, these results suggest that MscL self-assembly into channel clusters plays an osmoregulatory functional role in the membrane.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherCell Press
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1252
dc.relation.ispartofpageto1260
dc.relation.ispartofissue5
dc.relation.ispartofjournalBiophysical Journal
dc.relation.ispartofvolume100
dc.rights.retentionY
dc.subject.fieldofresearchPhysical sciences
dc.subject.fieldofresearchBiological physics
dc.subject.fieldofresearchChemical sciences
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchcode51
dc.subject.fieldofresearchcode510501
dc.subject.fieldofresearchcode34
dc.subject.fieldofresearchcode31
dc.titleBilayer-Mediated Clustering and Functional Interaction of MscL Channels
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2011
gro.hasfulltextNo Full Text
gro.griffith.authorBattle, Andrew


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