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dc.contributor.authorHe, JZ
dc.contributor.authorXu, ZH
dc.contributor.authorHughes, J
dc.date.accessioned2017-05-03T11:31:53Z
dc.date.available2017-05-03T11:31:53Z
dc.date.issued2005
dc.date.modified2009-09-02T07:42:08Z
dc.identifier.issn0378-1097
dc.identifier.doi10.1016/j.femsle.2005.04.033
dc.identifier.urihttp://hdl.handle.net/10072/4312
dc.description.abstractSoil fungal communities were studied using 18S rDNA-based molecular techniques. Soil DNA was analyzed using temperature gradient gel electrophoresis (TGGE), single-stranded conformational polymorphism (SSCP), cloning and sequencing methods, following community DNA extraction and polymerase chain reaction (PCR). The extracted community DNA was successfully amplified using the primer pair of EF4f-Fung5r which produced ca. 550 bp 18S rDNA fragments. TGGE screening of the PCR products showed some differences in band position and intensity between two soil samples in adjacent natural forest (YNF) and hoop pine plantation (YHP) ecosystems at Yarraman in subtropical Australia. TGGE and SSCP could be used for screening PCR products. However, care must be exercised when interpreting the TGGE and SSCP results with respect to microbial diversity, because one band may not necessarily represent one species. It is recommended that the PCR products should be purified before TGGE or SSCP screening. SSCP screening of the clone sequences revealed differences among the clones. Sequence and phylogenetic analyses revealed that all obtained clones were affiliated to the kingdom Fungi, including three phyla, i.e., Zygomycota, Ascomycota and Basidiomycota. Our results suggested that community DNA extraction, PCR, cloning, SSCP screening of clones, sequencing of selected clones and phylogentic analyses could be a good strategy in investigation of soil fungal community and diversity.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier Science - Blackwell Publishing
dc.publisher.placeAmsterdam, Netherlands /UK
dc.publisher.urihttp://www.blackwell-synergy.com/doi/full/10.1016/j.femsle.2005.04.033
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom91
dc.relation.ispartofpageto100
dc.relation.ispartofjournalFEMS Microbiology Letters
dc.relation.ispartofvolume247
dc.rights.retentionY
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchAgricultural, veterinary and food sciences
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode30
dc.subject.fieldofresearchcode32
dc.titleAnalyses of soil fungal communities in adjacent natural forest and hoop pine plantation ecosystems of subtropical Australia using molecular approaches based on 18S rRNA genes
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Sciences, Griffith School of Environment
gro.rights.copyright© 2005 Blackwell Publishing. The definitive version is available at www.interscience.wiley.com
gro.date.issued2005
gro.hasfulltextNo Full Text
gro.griffith.authorHughes, Jane M.
gro.griffith.authorXu, Zhihong


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