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  • Association of MiR-126 with Soluble Mesothelin-Related Peptides, a Marker for Malignant Mesothelioma

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    Author(s)
    Santarelli, Lory
    Strafella, Elisabetta
    Staffolani, Sara
    Amati, Monica
    Emanuelli, Monica
    Sartini, Davide
    Pozzi, Valentina
    Carbonari, Damiano
    Bracci, Massimo
    Pignotti, Elettra
    Mazzanti, Paola
    Sabbatini, Armando
    Ranaldi, Renzo
    Gasparini, Stefano
    Neuzil, Jiri
    Tomasetti, Marco
    Griffith University Author(s)
    Neuzil, Jiri
    Year published
    2011
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    Abstract
    Background Improved detection methods for diagnosis of malignant pleural mesothelioma (MPM) are essential for early and reliable detection as well as treatment. Since recent data point to abnormal levels of microRNAs (miRNAs) in tumors, we hypothesized that a profile of deregulated miRNAs may be a marker of MPM and that the levels of specific miRNAs may be used for monitoring its progress. Methods and Results miRNAs isolated from fresh-frozen biopsies of MPM patients were tested for the expression of 88 types of miRNA involved in cancerogenesis. Most of the tested miRNAs were downregulated in the malignant tissues ...
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    Background Improved detection methods for diagnosis of malignant pleural mesothelioma (MPM) are essential for early and reliable detection as well as treatment. Since recent data point to abnormal levels of microRNAs (miRNAs) in tumors, we hypothesized that a profile of deregulated miRNAs may be a marker of MPM and that the levels of specific miRNAs may be used for monitoring its progress. Methods and Results miRNAs isolated from fresh-frozen biopsies of MPM patients were tested for the expression of 88 types of miRNA involved in cancerogenesis. Most of the tested miRNAs were downregulated in the malignant tissues compared with the normal tissues. Of eight significantly downregulated, three miRNAs were assayed in cancerous tissue and adjacent non-cancerous tissue sample pairs collected from 27 formalin-fixed, paraffin-embedded MPM tissues by quantitative RT-PCR. Among the miRNAs tested, only miR-126 significantly remained downregulated in the malignant tissues. Furthermore, the performance of the selected miR-126 as biomarker was evaluated in serum samples of asbestos-exposed subjects and MPM patients and compared with controls. MiR-126 was not affected by asbestos exposure, whereas it was found strongly associated with VEGF serum levels. Levels of miR-126 in serum, and its levels in patients' serum in association with a specific marker of MPM, SMRPs, correlate with subjects at high risk to develop MPM. Conclusions and Significance We propose miR-126, in association with SMRPs, as a marker for early detection of MPM. The identification of tumor biomarkers used alone or, in particular, in combination could greatly facilitate the surveillance procedure for cohorts of subjects exposed to asbestos.
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    Journal Title
    PloS One
    Volume
    6
    Issue
    4
    DOI
    https://doi.org/10.1371/journal.pone.0018232
    Copyright Statement
    © 2011 Santarelli et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)
    Subject
    Cancer cell biology
    Publication URI
    http://hdl.handle.net/10072/43218
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    • Journal articles

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