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dc.contributor.authorSantarelli, Lory
dc.contributor.authorStrafella, Elisabetta
dc.contributor.authorStaffolani, Sara
dc.contributor.authorAmati, Monica
dc.contributor.authorEmanuelli, Monica
dc.contributor.authorSartini, Davide
dc.contributor.authorPozzi, Valentina
dc.contributor.authorCarbonari, Damiano
dc.contributor.authorBracci, Massimo
dc.contributor.authorPignotti, Elettra
dc.contributor.authorMazzanti, Paola
dc.contributor.authorSabbatini, Armando
dc.contributor.authorRanaldi, Renzo
dc.contributor.authorGasparini, Stefano
dc.contributor.authorNeuzil, Jiri
dc.contributor.authorTomasetti, Marco
dc.date.accessioned2017-05-03T14:37:52Z
dc.date.available2017-05-03T14:37:52Z
dc.date.issued2011
dc.date.modified2012-05-17T22:12:32Z
dc.identifier.issn1932-6203
dc.identifier.doi10.1371/journal.pone.0018232
dc.identifier.urihttp://hdl.handle.net/10072/43218
dc.description.abstractBackground Improved detection methods for diagnosis of malignant pleural mesothelioma (MPM) are essential for early and reliable detection as well as treatment. Since recent data point to abnormal levels of microRNAs (miRNAs) in tumors, we hypothesized that a profile of deregulated miRNAs may be a marker of MPM and that the levels of specific miRNAs may be used for monitoring its progress. Methods and Results miRNAs isolated from fresh-frozen biopsies of MPM patients were tested for the expression of 88 types of miRNA involved in cancerogenesis. Most of the tested miRNAs were downregulated in the malignant tissues compared with the normal tissues. Of eight significantly downregulated, three miRNAs were assayed in cancerous tissue and adjacent non-cancerous tissue sample pairs collected from 27 formalin-fixed, paraffin-embedded MPM tissues by quantitative RT-PCR. Among the miRNAs tested, only miR-126 significantly remained downregulated in the malignant tissues. Furthermore, the performance of the selected miR-126 as biomarker was evaluated in serum samples of asbestos-exposed subjects and MPM patients and compared with controls. MiR-126 was not affected by asbestos exposure, whereas it was found strongly associated with VEGF serum levels. Levels of miR-126 in serum, and its levels in patients' serum in association with a specific marker of MPM, SMRPs, correlate with subjects at high risk to develop MPM. Conclusions and Significance We propose miR-126, in association with SMRPs, as a marker for early detection of MPM. The identification of tumor biomarkers used alone or, in particular, in combination could greatly facilitate the surveillance procedure for cohorts of subjects exposed to asbestos.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent707384 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherPublic Library of Science
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrome18232-1
dc.relation.ispartofpagetoe18232-9
dc.relation.ispartofissue4
dc.relation.ispartofjournalPloS One
dc.relation.ispartofvolume6
dc.rights.retentionY
dc.subject.fieldofresearchCancer cell biology
dc.subject.fieldofresearchcode321101
dc.titleAssociation of MiR-126 with Soluble Mesothelin-Related Peptides, a Marker for Malignant Mesothelioma
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://www.plos.org/journals/license.html
gro.rights.copyright© 2011 Santarelli et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)
gro.date.issued2011
gro.hasfulltextFull Text
gro.griffith.authorNeuzil, Jiri


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