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dc.contributor.authorGrayson, Jane E
dc.contributor.authorGrant, Gary D
dc.contributor.authorDukie, Shailendra
dc.contributor.authorVertullo, Christopher J
dc.date.accessioned2017-05-03T14:33:37Z
dc.date.available2017-05-03T14:33:37Z
dc.date.issued2011
dc.date.modified2012-03-01T22:12:38Z
dc.identifier.issn0009-921X
dc.identifier.doi10.1007/s11999-011-1768-3
dc.identifier.urihttp://hdl.handle.net/10072/43220
dc.description.abstractBackground Infection after ACL reconstruction is uncommon but catastrophic. Prophylactic graft saturation in vancomycin reportedly reduces infection rates. Questions/purposes We characterized vancomycin elution from soaked tendons. Specifically, the effect of rinsing was studied. We also determined how vancomycin concentration in the soak solution and tendon dimension influenced this elution rate, and examined whether the vancomycin amount released was lower than osteoblast and chondroblast toxic concentrations. Methods Bovine tendons were wrapped in sterile gauze swabs presoaked with 5-, 2.5-, or 1.25-mg/mL vancomycin solutions. After 10 minutes, rinsed and unrinsed tendons were placed in 100 mL agitated 37àphosphate-buffered saline (PBS). One-milliliter samples taken at 10 minutes and 1, 6, 12, 24, and 72 hours were analyzed by high-performance liquid chromatography. Results The maximum elution rate occurred between 10 minutes and 1 hour, with no lag between experiment initiation and drug appearance in the solution. Rinsing affected the initial amount in solution but had little influence on drug release after 10 minutes. Vancomycin diffusion rates were dependent on soak solution concentration at all sampling intervals. The vancomycin amount released or eluted did not increase after the 1-hour interval. At 24 hours, concentrations were 45 ᠱ2, 16 ᠱ, and 9 ᠳ 姯mL for the 5-, 2.5-, and 1.25-mg/mL solutions, respectively. Higher elution rates were observed in larger-volume tendons. Conclusions Soaked tendon grafts can act as reservoirs for vancomycin, with the amount released and elution profile dependent on rinsing, tendon volume, and soak solution concentration. Vancomycin elution was lower than previously reported osteoblast and chondroblast toxicity concentrations and above the minimum inhibitory concentration for Staphylococcus. Clinical Relevance Presoaking ACL reconstruction autografts with vancomycin may reduce the risk of ACL reconstruction infection without the risk of local or general toxicity.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer New York LLC
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom2948
dc.relation.ispartofpageto2952
dc.relation.ispartofissue10
dc.relation.ispartofjournalClinical Orthopaedics and Related Research
dc.relation.ispartofvolume469
dc.rights.retentionY
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchPharmacology and pharmaceutical sciences not elsewhere classified
dc.subject.fieldofresearchcode3202
dc.subject.fieldofresearchcode321499
dc.titleThe In Vitro Elution Characteristics of Vancomycin from Tendons
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Rehabilitation Sciences
gro.date.issued2011
gro.hasfulltextNo Full Text
gro.griffith.authorGrant, Gary D.
gro.griffith.authorAnoopkumar-Dukie, Shailendra


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