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  • Directed evolution of efficient secretion in the SRP-dependent export of TolB

    Author(s)
    Zalucki, Yaramah M
    Shafer, William M
    Jennings, Michael P
    Griffith University Author(s)
    Jennings, Michael P.
    Year published
    2011
    Metadata
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    Abstract
    Signal sequence non-optimal codons have been shown to be important for the folding and efficient export of maltose binding protein (MBP), a SecB dependent protein. In this study, we analysed the importance of signal sequence non-optimal codons of TolB, a signal recognition particle (SRP) dependent exported protein. The protein production levels of wild type TolB (TolB-wt) and a mutant allele of TolB in which all signal sequence non-optimal codons were changed to a synonymous optimal codon (TolB-opt), revealed that TolB-opt production was 12-fold lower than TolB-wt. This difference could not be explained by changes in ...
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    Signal sequence non-optimal codons have been shown to be important for the folding and efficient export of maltose binding protein (MBP), a SecB dependent protein. In this study, we analysed the importance of signal sequence non-optimal codons of TolB, a signal recognition particle (SRP) dependent exported protein. The protein production levels of wild type TolB (TolB-wt) and a mutant allele of TolB in which all signal sequence non-optimal codons were changed to a synonymous optimal codon (TolB-opt), revealed that TolB-opt production was 12-fold lower than TolB-wt. This difference could not be explained by changes in mRNA levels, or plasmid copy number, which was the same in both strains. A directed evolution genetic screen was used to select for mutants in the TolB-opt signal sequence that resulted in higher levels of TolB production. Analysis of the 46 independent TolB mutants that reverted to wild type levels of expression revealed that at least four signal sequence non-optimal codons were required. These results suggest that non-optimal codons may be required for the folding and efficient export of all proteins exported via the Sec system, regardless of whether they are dependent on SecB or SRP for delivery to the inner membrane.
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    Journal Title
    Biochimica et Biophysica Acta-Biomembranes
    Volume
    1808
    Issue
    10
    DOI
    https://doi.org/10.1016/j.bbamem.2011.06.004
    Subject
    Biochemistry and cell biology
    Other biological sciences
    Chemical engineering
    Medical microbiology not elsewhere classified
    Publication URI
    http://hdl.handle.net/10072/43392
    Collection
    • Journal articles

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