Show simple item record

dc.contributor.authorJennings, Michaelen_US
dc.contributor.authorM. Shafer, Williamen_US
dc.contributor.authorM. Zalucki, Yaramahen_US
dc.date.accessioned2017-04-04T17:15:25Z
dc.date.available2017-04-04T17:15:25Z
dc.date.issued2011en_US
dc.date.modified2012-03-07T05:38:20Z
dc.identifier.issn00052736en_US
dc.identifier.doi10.1016/j.bbamem.2011.06.004en_US
dc.identifier.urihttp://hdl.handle.net/10072/43392
dc.description.abstractSignal sequence non-optimal codons have been shown to be important for the folding and efficient export of maltose binding protein (MBP), a SecB dependent protein. In this study, we analysed the importance of signal sequence non-optimal codons of TolB, a signal recognition particle (SRP) dependent exported protein. The protein production levels of wild type TolB (TolB-wt) and a mutant allele of TolB in which all signal sequence non-optimal codons were changed to a synonymous optimal codon (TolB-opt), revealed that TolB-opt production was 12-fold lower than TolB-wt. This difference could not be explained by changes in mRNA levels, or plasmid copy number, which was the same in both strains. A directed evolution genetic screen was used to select for mutants in the TolB-opt signal sequence that resulted in higher levels of TolB production. Analysis of the 46 independent TolB mutants that reverted to wild type levels of expression revealed that at least four signal sequence non-optimal codons were required. These results suggest that non-optimal codons may be required for the folding and efficient export of all proteins exported via the Sec system, regardless of whether they are dependent on SecB or SRP for delivery to the inner membrane.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.languageEnglishen_US
dc.publisherElsevier BVen_US
dc.publisher.placeNetherlandsen_US
dc.relation.ispartofstudentpublicationNen_US
dc.relation.ispartofpagefrom2544en_US
dc.relation.ispartofpageto2550en_US
dc.relation.ispartofissue10en_US
dc.relation.ispartofjournalBiochimica et Biophysica Acta-Biomembranesen_US
dc.relation.ispartofvolume1808en_US
dc.rights.retentionYen_US
dc.subject.fieldofresearchMedical Microbiology not elsewhere classifieden_US
dc.subject.fieldofresearchcode110899en_US
dc.titleDirected evolution of efficient secretion in the SRP-dependent export of TolBen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2011
gro.hasfulltextNo Full Text


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record