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dc.contributor.authorTiralongo, Joeen_US
dc.contributor.authorMaggioni, Andreaen_US
dc.date.accessioned2017-04-24T11:32:13Z
dc.date.available2017-04-24T11:32:13Z
dc.date.issued2011en_US
dc.date.modified2013-05-14T00:28:32Z
dc.identifier.issn10643745en_US
dc.identifier.doi10.1007/978-1-61737-967-3_14en_US
dc.identifier.urihttp://hdl.handle.net/10072/43425
dc.description.abstractThe heterologous expression of functional mammalian integral membrane proteins still represents a significant hurdle towards evaluating the relationship between their structure and function. We have therefore utilised the OmpA signal sequence to deliberately target the expression of a mammalian nucleotide sugar transporter, the murine CMP-sialic acid transporter, to the E. coli inner membrane. The functionality of the recombinant CMP-sialic acid transporter could then be evaluated either following the spheroplasting of E. coli cells or through the isolation of the E. coli inner membrane and the formation of mixed phosphatidylcholine-inner membrane proteoliposomes.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.format.extent414807 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglishen_US
dc.language.isoen_US
dc.publisherSpringeren_US
dc.publisher.placeUnited Statesen_US
dc.relation.ispartofstudentpublicationNen_US
dc.relation.ispartofpagefrom237en_US
dc.relation.ispartofpageto249en_US
dc.relation.ispartofjournalMethods in Molecular Biologyen_US
dc.relation.ispartofvolume705en_US
dc.rights.retentionYen_US
dc.subject.fieldofresearchReceptors and Membrane Biologyen_US
dc.subject.fieldofresearchcode060110en_US
dc.titleThe Targeted Expression of Nucleotide Sugar Transporters to the E. coli Inner Membraneen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.facultyOffice of the Snr Dep Vice Chancellor, Institute for Glycomicsen_US
gro.rights.copyrightCopyright 2011 Springer. This is an electronic version of an article published in Methods in Molecular Biology, Vol. 705, 2011, pp. 237-249. Methods in Molecular Biology is available online at: http://link.springer.com// with the open URL of your article.en_US
gro.date.issued2011
gro.hasfulltextFull Text


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