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dc.contributor.authorArndt, Nadiaen_US
dc.contributor.authorDay, Christopheren_US
dc.contributor.authorMadge, Paulen_US
dc.contributor.authorTiralongo, Joeen_US
dc.contributor.authorvon Itzstein, Marken_US
dc.date.accessioned2017-04-04T22:36:26Z
dc.date.available2017-04-04T22:36:26Z
dc.date.issued2011en_US
dc.date.modified2012-03-08T22:32:45Z
dc.identifier.issn07302312en_US
dc.identifier.doi10.1002/jcb.23139en_US
dc.identifier.urihttp://hdl.handle.net/10072/43447
dc.description.abstractCurrently there is only a modest level knowledge of the glycosylation status of immortalised cell lines that are commonly used in cancer biology as well as their binding affinities to different glycan structures. Through use of glycan and lectin microarray technology, this study has endeavoured to define the different bindings of cell surface carbohydrate structures to glycan-binding lectins. The screening of breast cancer MDA-MB435 cells, cervical cancer HeLa cells and colon cancer Caco-2, HCT116 and HCT116-FM6 cells was conducted to determine their differential bindings to a variety of glycan and lectin structures printed on the array slides. An inverse relationship between the number of glycan structures recognised and the variety of cell surface glycosylation was observed. Of the cell lines tested, it was found that four bound to sialylated structures in initial screening. Secondary screening in the presence of a neuraminidase inhibitor (4-deoxy-4-guanidino- Neu5Ac2en) significantly reduced sialic acid binding. The array technology has proven to be useful in determining the glycosylation signatures of various cell-lines as well as their glycan binding preferences. The findings of this study provide the groundwork for further investigation into the numerous glycan-lectin interactions that are exhibited by immortalised cell lines.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.languageEnglishen_US
dc.publisherJohn Wiley & Sons Inc.en_US
dc.publisher.placeUnited Statesen_US
dc.relation.ispartofstudentpublicationYen_US
dc.relation.ispartofpagefrom2230en_US
dc.relation.ispartofpageto2240en_US
dc.relation.ispartofissue9en_US
dc.relation.ispartofjournalJournal of Cellular Biochemistryen_US
dc.relation.ispartofvolume112en_US
dc.rights.retentionYen_US
dc.subject.fieldofresearchCellular Interactions (incl. Adhesion, Matrix, Cell Wall)en_US
dc.subject.fieldofresearchReceptors and Membrane Biologyen_US
dc.subject.fieldofresearchCancer Cell Biologyen_US
dc.subject.fieldofresearchcode060106en_US
dc.subject.fieldofresearchcode060110en_US
dc.subject.fieldofresearchcode111201en_US
dc.titleDifferential Carbohydrate Binding and Cell Surface Glycosylation of Human Cancer Cell Linesen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.facultyOffice of the Snr Dep Vice Chancellor, Institute for Glycomicsen_US
gro.date.issued2011
gro.hasfulltextNo Full Text


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