Show simple item record

dc.contributor.authorAlexander, Kylie
dc.contributor.authorChang, Ming
dc.contributor.authorMaylin, Erin
dc.contributor.authorKohler, Thomas
dc.contributor.authorMüller, Ralph
dc.contributor.authorWu, Andy
dc.contributor.authorVan Rooijen, Nico
dc.contributor.authorSweet, Matthew
dc.contributor.authorHume, David
dc.contributor.authorRaggatt, Liza
dc.contributor.authorPettit, Allison
dc.date.accessioned2017-05-03T14:38:03Z
dc.date.available2017-05-03T14:38:03Z
dc.date.issued2011
dc.date.modified2012-03-20T23:06:03Z
dc.identifier.issn08840431
dc.identifier.doi10.1002/jbmr.354
dc.identifier.urihttp://hdl.handle.net/10072/43715
dc.description.abstractBone-lining tissues contain a population of resident macrophages termed osteomacs that interact with osteoblasts in vivo and control mineralization in vitro. The role of osteomacs in bone repair was investigated using a mouse tibial bone injury model that heals primarily through intramembranous ossification and progresses through all major phases of stabilized fracture repair. Immunohistochemical studies revealed that at least two macrophage populations, F4/80 Mac-2 /lowTRACP osteomacs and F4/80 Mac-2hiTRACP inflammatory macrophages, were present within the bone injury site and persisted throughout the healing time course. In vivo depletion of osteomacs/macrophages (either using the Mafia transgenic mouse model or clodronate liposome delivery) or osteoclasts (recombinant osteoprotegerin treatment) established that osteomacs were required for deposition of collagen type 1 (CT1 ) matrix and bone mineralization in the tibial injury model, as assessed by quantitative immunohistology and micro-computed tomography. Conversely, administration of the macrophage growth factor colony-stimulating factor 1 (CSF-1) increased the number of osteomacs/macrophages at the injury site significantly with a concurrent increase in new CT1 matrix deposition and enhanced mineralization. This study establishes osteomacs as participants in intramembranous bone healing and as targets for primary anabolic bone therapies.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1517
dc.relation.ispartofpageto1532
dc.relation.ispartofissue7
dc.relation.ispartofjournalJournal of Bone and Mineral Research
dc.relation.ispartofvolume26
dc.rights.retentionY
dc.subject.fieldofresearchImmunology not elsewhere classified
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchEngineering
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode110799
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode09
dc.subject.fieldofresearchcode11
dc.titleOsteal macrophages promote in vivo intramembranous bone healing in a mouse tibial injury model
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2011
gro.hasfulltextNo Full Text
gro.griffith.authorWu, Andy C.


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record