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dc.contributor.authorLiu, Qin
dc.contributor.authorHumpe, Andreas
dc.contributor.authorKletsas, Dimitris
dc.contributor.authorWarnke, Frauke
dc.contributor.authorBecker, Stephan T
dc.contributor.authorDouglas, Timothy
dc.contributor.authorSivananthan, Sureshan
dc.contributor.authorWarnke, Patrick H
dc.date.accessioned2017-10-26T01:32:24Z
dc.date.available2017-10-26T01:32:24Z
dc.date.issued2011
dc.date.modified2014-09-08T22:08:59Z
dc.identifier.issn0882-2786
dc.identifier.urihttp://hdl.handle.net/10072/44070
dc.description.abstractAbstract PURPOSE: Human mesenchymal stem cells (hMSCs) hold the potential for bone regeneration because of their self-renewing and multipotent character. The goal of this study was to evaluate the influence of collagen membranes on the proliferation of hMSCs derived from bone marrow. A special focus was set on short-term eluates derived from collagen membranes, as volatile toxic materials washed out from these membranes may influence cell behavior during the short time course of oral surgery. MATERIALS AND METHODS: The proliferation of hMSCs seeded directly on a collagen membrane (BioGide) was evaluated quantitatively using the cell proliferation reagent WST-1 (4-3-[4-iodophenyl]-2-[4-nitrophenyl]-2H-[5-tetrazolio]-1, 3--benzol-disulfonate) and qualitatively by scanning electron microscopy. Two standard biocompatibility tests, namely the lactate dehydrogenase and MTT (3-[4, 5-dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazoliumbromide) tests, were performed using hMSCs cultivated in eluates from membranes incubated for 10 minutes, 1 hour, or 24 hours in serum-free cell culture medium. The data were analyzed statistically. RESULTS: Scanning electron microscopy showed large numbers of hMSCs with well-spread morphology on the collagen membranes after 7 days of culture. The WST test revealed significantly better proliferation of hMSCs on collagen membranes after 4 days of culture compared to cells cultured on a cover glass. Cytotoxicity levels were low, peaking in short-term eluates and decreasing with longer incubation times. CONCLUSION: Porcine collagen membranes showed good biocompatibility in vitro for hMSCs. If maximum cell proliferation rates are required, a prewash of membranes prior to application may be useful.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherQUINTESSENCE
dc.publisher.placeUnited Kingdom
dc.publisher.urihttp://www.quintpub.com/journals/omi/abstract.php?article_id=11366#.U6DJdbEudZI
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1004
dc.relation.ispartofpageto1010
dc.relation.ispartofissue5
dc.relation.ispartofjournalInternational Journal of Oral & Maxillofacial Implants
dc.relation.ispartofvolume26
dc.rights.retentionY
dc.subject.fieldofresearchOral and Maxillofacial Surgery
dc.subject.fieldofresearchMedical Physics
dc.subject.fieldofresearchBiomedical Engineering
dc.subject.fieldofresearchDentistry
dc.subject.fieldofresearchcode110504
dc.subject.fieldofresearchcode029903
dc.subject.fieldofresearchcode0903
dc.subject.fieldofresearchcode1105
dc.titleProliferation Assessment of primary human mesenchymal stem cells on collagen membranes for guided bone regeneration
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyrightSelf-archiving of the author-manuscript version is not yet supported by this journal. Please refer to the journal link for access to the definitive, published version or contact the authors for more information.
gro.date.issued2011
gro.hasfulltextNo Full Text
gro.griffith.authorWarnke, Frauke
gro.griffith.authorWarnke, Patrick H.


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