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dc.contributor.authorKidd, Stephen P.en_US
dc.contributor.authorY. Djoko, Karreraen_US
dc.contributor.authorNg, JiaQien_US
dc.contributor.authorPilar Argente, M.en_US
dc.contributor.authorJennings, Michaelen_US
dc.contributor.authorG. McEwan, Alastairen_US
dc.date.accessioned2017-04-24T10:18:45Z
dc.date.available2017-04-24T10:18:45Z
dc.date.issued2011en_US
dc.date.modified2012-04-01T22:39:43Z
dc.identifier.issn17565901en_US
dc.identifier.doi10.1039/C1MT00127Ben_US
dc.identifier.urihttp://hdl.handle.net/10072/44113
dc.description.abstractWe have identified a novel regulator from the MerR family of transcription factors in the bacterial pathogen Haemophilus influenzae (HI1623; nickel-associated merR-like Regulator - NimR). NimR regulates the expression of a Ni2+ uptake transporter (NikKLMQO). The promoters for nimR and the nik operon are divergent and overlapping and NimR binds at a site between the promoter elements for nikKLMQO. Expression of this operon requires NimR and depends on Ni2+. Growth rates of the H. influenzae nimR and nikQ mutants were reduced in chemically defined media compared to the wild type and the mutants were unable to grow in the presence of EDTA. The mutant strains were less tolerant of acidic pH and the wild type Rd KW20 could not tolerate low pH in the presence of fluoramide, a urease specific inhibitor, confirming that both nickel transport and urea hydrolysis are a central process in pH control. H. influenzae nimR and nikQ strains were deficient in urease activity, but this could be specifically restored by the addition of excess Ni2+. NimR did not directly regulate the expression of urease genes but the activity of urease requires both nimR and nikQ. Purified NimR is a dimer that binds 1 Ni2+ion. NimR is the first example of a Ni-dependent regulator from the MerR family and targeting a metal ion uptake system; it is distinct from NikR the Ni-responsive regulators of the ribbon-helix-helix family.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.languageEnglishen_US
dc.publisherRoyal Society of Chemistryen_US
dc.publisher.placeUnited Kingdomen_US
dc.relation.ispartofstudentpublicationNen_US
dc.relation.ispartofpagefrom1009en_US
dc.relation.ispartofpageto1018en_US
dc.relation.ispartofissue10en_US
dc.relation.ispartofjournalMetallomics: integrated biometal scienceen_US
dc.relation.ispartofvolume3en_US
dc.rights.retentionYen_US
dc.subject.fieldofresearchMedical Microbiology not elsewhere classifieden_US
dc.subject.fieldofresearchcode110899en_US
dc.titleA novel nickel responsive MerR-like regulator, NimR, from Haemophilus influenzaeen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2011
gro.hasfulltextNo Full Text


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