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dc.contributor.authorIvanovski, Sasoen_US
dc.contributor.authorHamlet, Stephenen_US
dc.contributor.authorRetzepi, M.en_US
dc.contributor.authorWall, I.en_US
dc.contributor.authorDonos, Nen_US
dc.date.accessioned2017-05-03T12:27:30Z
dc.date.available2017-05-03T12:27:30Z
dc.date.issued2011en_US
dc.date.modified2012-04-09T22:56:31Z
dc.identifier.issn09057161en_US
dc.identifier.doi10.1111/j.1600-0501.2010.02104.xen_US
dc.identifier.urihttp://hdl.handle.net/10072/44289
dc.description.abstractObjectives: Guided bone regeneration (GBR) is a commonly utilized surgical technique in the craniofacial region. The transcriptional mechanisms associated with this type of bone regeneration are not well understood. The aim of this study was to characterize the transcriptome associated with GBR of a critical-size calvarial defect in the rat. Material and methods: Critical-size calvarial defects were created in six Wistar strain rats and treated according to the principles of GBR. The tissue filling the regenerating defect was harvested at 7 and 14 days. Total RNA was extracted and microarray analysis was carried out to identify the differences in the transcriptome between days 7 and 14. Results: Gene ontology (GO) analysis of the genes up-regulated at day 7 showed that immature wound healing-related mechanisms, such as protein metabolism and cell proliferation, were upregulated at this time point. Furthermore, the immuno-inflammatory process was also up-regulated at the earlier time point. In contrast, by day 14, GO groups consistent with wound maturation, such as extracellular matrix formation, anatomical structure development and cell differentiation, were upregulated. Furthermore, the functionally important GO categories of skeletal development, ossification and bone mineralization were up-regulated at day 14. Genes of interest that belonged to this group and were up-regulated at day 14 included growth and differentiation factors (Bmp2, Bmp3, Tgfb3), extracellular matrix proteins (osteocalcin, osteomodulin, stenniocalcin 1) and transcription factors (Runx2, Sox6, Satb2). Furthermore, a number of genes associated with Tgfb/Bmp and Wnt signalling were also up-regulated. Besides skeletogenesis, genes associated with angiogenesis and neurogenesis were also up-regulated at day 14. Conclusions: The transcriptome associated with a maturing GBR-treated craniofacial bone defect is characterized by the down-regulation of the immuno-inflammatory response and up-regulation of skeletogeneis-, angiogenesis- and neurogenesis-associated genes. The Tgfb/Bmp and Wnt signalling pathways play an important role in the regenerative process.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.languageEnglishen_US
dc.publisherWiley-Blackwell Publishing, Inc.en_US
dc.publisher.placeUnited Statesen_US
dc.relation.ispartofstudentpublicationNen_US
dc.relation.ispartofpagefrom382en_US
dc.relation.ispartofpageto389en_US
dc.relation.ispartofissue4en_US
dc.relation.ispartofjournalClinical Oral Implants Researchen_US
dc.relation.ispartofvolume22en_US
dc.rights.retentionYen_US
dc.subject.fieldofresearchBiomedical Engineering not elsewhere classifieden_US
dc.subject.fieldofresearchcode090399en_US
dc.titleTranscriptional profiling of "guided bone regeneration" in a critical-size calvarial defecten_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.facultyGriffith Health, School of Dentistry and Oral Healthen_US
gro.date.issued2011
gro.hasfulltextNo Full Text


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