Show simple item record

dc.contributor.authorOgg, Christopher D
dc.contributor.authorPatel, Bharat KC
dc.date.accessioned2017-05-08T12:30:23Z
dc.date.available2017-05-08T12:30:23Z
dc.date.issued2011
dc.date.modified2012-04-09T22:57:12Z
dc.identifier.issn1466-5026
dc.identifier.doi10.1099/ijs.0.023655-0
dc.identifier.urihttp://hdl.handle.net/10072/44290
dc.description.abstractA strictly thermophilic anaerobe, designated strain VF08T, was isolated from a water sample collected from a Great Artesian Basin bore (registered bore number 22981) situated at Mitchell, QLD, Australia. Cells of isolate VF08T were slightly curved, non-sporulating rods (1.5-3.5װ.4-0.8 孩, which stained Gram-negative but possessed a Gram-positive cell-wall ultrastructure. The strain grew optimally in tryptone-yeast extract-glucose (TYEG) medium at 55 à(temperature growth range between 37 and 60 é and a pH of 7 (pH growth range, 6.0-9.0). Yeast extract or tryptone was required for growth on glucose, fructose, xylose, maltose, sucrose, raffinose, cellobiose, ribose, pyruvate, tryptone, peptone, Casamino acids, amyl media and serine, but could also support growth as the sole carbon source. End products from glucose fermentation were acetate, ethanol, CO2 and H2. The strain reduced vanadium(V), but not iron(III), manganese(IV), elemental sulfur, sulfate, thiosulfate, sulfite, nitrate or nitrite in the presence of 0.2?% yeast extract, peptone, tryptone, glucose, sucrose and Casamino acids, but an increase in the growth rate or cell yield was not observed. Growth was inhibited by chloramphenicol, streptomycin, tetracycline, penicillin, ampicillin and =2?% NaCl (w/v). The G+C content of the DNA was 38.4ᰮ8 mol% as determined by the thermal denaturation (Tm) method. 16S rRNA gene sequence analysis revealed that isolate VF08T was a member of the genus Caloramator with Caloramator australicus and Caloramator fervidus (formerly Clostridium fervidus) being the closest relatives with similarity values of 85.0 and 86.1?%, respectively, when helix 6 nucleotides were included in the analysis, and 95.2?% and 94?%, respectively, when these nucleotides were masked from the analysis. Further analysis revealed that strain VF08T formed an individual cluster (cluster II) within the genus Caloramator and could be distinguished from other species within the genus Caloramator (clusters I, III and IV) on the basis of signature nucleotides and differences in phenotypic traits. These data suggest that strain VF08T is a novel species of the genus Caloramator, for which the name Caloramator mitchellensis sp. nov. is proposed. The type strain is VF08T (=JCM 15828T=KCTC 5735T). An emended description of the genus Caloramator is also provided.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSociety for General Microbiology
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom644
dc.relation.ispartofpageto653
dc.relation.ispartofissue3
dc.relation.ispartofjournalInternational Journal of Systematic and Evolutionary Microbiology
dc.relation.ispartofvolume61
dc.rights.retentionY
dc.subject.fieldofresearchMedical Microbiology not elsewhere classified
dc.subject.fieldofresearchMicrobiology not elsewhere classified
dc.subject.fieldofresearchEvolutionary Biology not elsewhere classified
dc.subject.fieldofresearchEvolutionary Biology
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchMedical Microbiology
dc.subject.fieldofresearchcode110899
dc.subject.fieldofresearchcode060599
dc.subject.fieldofresearchcode060399
dc.subject.fieldofresearchcode0603
dc.subject.fieldofresearchcode0605
dc.subject.fieldofresearchcode1108
dc.titleCaloramator mitchellensis sp. nov., a thermoanaerobe isolated from the geothermal waters of the Great Artesian Basin of Australia, and emended description of the genus Caloramator
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2011
gro.hasfulltextNo Full Text
gro.griffith.authorPatel, Bharat K.
gro.griffith.authorOgg, Christopher D.


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record