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dc.contributor.authorHamilton, S.en_US
dc.contributor.authorSippel, S.en_US
dc.contributor.authorBunn, Stuarten_US
dc.contributor.editorPaul F. Kempen_US
dc.date.accessioned2017-05-03T11:34:09Z
dc.date.available2017-05-03T11:34:09Z
dc.date.issued2005en_US
dc.date.modified2009-12-04T05:22:34Z
dc.identifier.issn15415856en_US
dc.identifier.doi10.4319/lom.2005.3.149en_US
dc.identifier.urihttp://hdl.handle.net/10072/4431
dc.description.abstractStable isotope and ecological stoichiometry investigations of aquatic food webs require separate measurements of microalgae and detritus, but fine particulate matter collected as seston or scraped from biofilms typically is an unknown mixture of these two components plus other material. This paper describes an economical method to partition fine particulate matter into predominantly algal and detrital components by centrifugation in colloidal silica. Centrifugation using this density also worked well to separate algae from inorganic matter. The protocol was tested by sampling a broad range of water bodies in Queensland, Australia. Seston was concentrated using an inexpensive continuous centrifuge and organic matter was collected from surfaces of rocks, mud, or plants. Separation in colloidal silica was achieved using a standard benchtop centrifuge. When the colloidal silica was adjusted to a density of 1.27 g/cm3, the light fraction (supernatant) tended to be dominated by algal organic matter while the heavy fraction tended to be more detrital. Ratios of organic carbon to chlorophyll a indicated the efficacy of the separations. Stable C and N isotope ratios often differed considerably between the algal and detrital fractions, demonstrating the need to perform such separations in many aquatic environments.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherAmerican Society for Limnology and Oceanographyen_US
dc.publisher.placeWaco Texas USAen_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom149en_US
dc.relation.ispartofpageto157en_US
dc.relation.ispartofjournalLimnology and Oceanography: Methodsen_US
dc.relation.ispartofvolume3en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchcode270701en_US
dc.titleSeparation of algae from detritus for stable isotope or ecological stoichiometry studies using density fractionation in colloidal silicaen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2005
gro.hasfulltextNo Full Text


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