Phosphoinositide 3-Kinase (PI3K(p110α)) Directly Regulates Key Components of the Z-disc and Cardiac Structure
Author(s)
Waardenberg, Ashley
Bernardo, Bianca C.
Ng, Dominic C. H.
Shepherd, Peter R.
Cemerlang, Nelly
Sbroggio, Mauro
Wells, Christine
Dalrymple, Brian P.
Brancaccio, Mara
Lin, Ruby C. Y.
McMullen, Julie R.
Year published
2011
Metadata
Show full item recordAbstract
Maintenance of cardiac structure and Z-disc signaling are key factors responsible for protecting the heart in a setting of stress, but how these processes are regulated is not well defined. We recently demonstrated that PI3K(p110a) protects the heart against myocardial infarction. The aim of this study was to determine whether PI3K(p110a) directly regulates components of the Z-disc and cardiac structure. To address this question, a unique three-dimensional virtual muscle model was applied to gene expression data from transgenic mice with increased or decreased PI3K(p110a) activity under basal conditions (sham) and ...
View more >Maintenance of cardiac structure and Z-disc signaling are key factors responsible for protecting the heart in a setting of stress, but how these processes are regulated is not well defined. We recently demonstrated that PI3K(p110a) protects the heart against myocardial infarction. The aim of this study was to determine whether PI3K(p110a) directly regulates components of the Z-disc and cardiac structure. To address this question, a unique three-dimensional virtual muscle model was applied to gene expression data from transgenic mice with increased or decreased PI3K(p110a) activity under basal conditions (sham) and in a setting of myocardial infarction to display the location of structural proteins. Key findings from this analysis were then validated experimentally. The three-dimensional virtual muscle model visually highlighted reciprocally regulated transcripts associated with PI3K activation that encoded key components of the Z-disc and costamere, including melusin. Studies were performed to assess whether PI3K and melusin interact in the heart. Here, we identify a novel melusin- PI3K interaction that generates lipid kinase activity. The direct impact of PI3K(p110a) on myocyte structure was assessed by treating neonatal rat ventricular myocytes with PI3K(p110a) inhibitors and examining the myofiber morphology of hearts from PI3K transgenic mice. Results demonstrate that PI3K is critical for myofiber maturation and Z-disc alignment. In summary, PI3K regulates the expression of genes essential for cardiac structure and Z-disc signaling, interacts with melusin, and is critical for Z-disc alignment.
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View more >Maintenance of cardiac structure and Z-disc signaling are key factors responsible for protecting the heart in a setting of stress, but how these processes are regulated is not well defined. We recently demonstrated that PI3K(p110a) protects the heart against myocardial infarction. The aim of this study was to determine whether PI3K(p110a) directly regulates components of the Z-disc and cardiac structure. To address this question, a unique three-dimensional virtual muscle model was applied to gene expression data from transgenic mice with increased or decreased PI3K(p110a) activity under basal conditions (sham) and in a setting of myocardial infarction to display the location of structural proteins. Key findings from this analysis were then validated experimentally. The three-dimensional virtual muscle model visually highlighted reciprocally regulated transcripts associated with PI3K activation that encoded key components of the Z-disc and costamere, including melusin. Studies were performed to assess whether PI3K and melusin interact in the heart. Here, we identify a novel melusin- PI3K interaction that generates lipid kinase activity. The direct impact of PI3K(p110a) on myocyte structure was assessed by treating neonatal rat ventricular myocytes with PI3K(p110a) inhibitors and examining the myofiber morphology of hearts from PI3K transgenic mice. Results demonstrate that PI3K is critical for myofiber maturation and Z-disc alignment. In summary, PI3K regulates the expression of genes essential for cardiac structure and Z-disc signaling, interacts with melusin, and is critical for Z-disc alignment.
View less >
Journal Title
Journal of Biological Chemistry
Volume
286
Issue
35
Subject
Gene Expression (incl. Microarray and other genome-wide approaches)
Chemical Sciences
Biological Sciences
Medical and Health Sciences