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  • Phosphoinositide 3-Kinase (PI3K(p110α)) Directly Regulates Key Components of the Z-disc and Cardiac Structure

    Author(s)
    Waardenberg, Ashley
    Bernardo, Bianca C.
    Ng, Dominic C. H.
    Shepherd, Peter R.
    Cemerlang, Nelly
    Sbroggio, Mauro
    Wells, Christine
    Dalrymple, Brian P.
    Brancaccio, Mara
    Lin, Ruby C. Y.
    McMullen, Julie R.
    Griffith University Author(s)
    Waardenberg, Ashley
    Wells, Christine
    Year published
    2011
    Metadata
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    Abstract
    Maintenance of cardiac structure and Z-disc signaling are key factors responsible for protecting the heart in a setting of stress, but how these processes are regulated is not well defined. We recently demonstrated that PI3K(p110a) protects the heart against myocardial infarction. The aim of this study was to determine whether PI3K(p110a) directly regulates components of the Z-disc and cardiac structure. To address this question, a unique three-dimensional virtual muscle model was applied to gene expression data from transgenic mice with increased or decreased PI3K(p110a) activity under basal conditions (sham) and ...
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    Maintenance of cardiac structure and Z-disc signaling are key factors responsible for protecting the heart in a setting of stress, but how these processes are regulated is not well defined. We recently demonstrated that PI3K(p110a) protects the heart against myocardial infarction. The aim of this study was to determine whether PI3K(p110a) directly regulates components of the Z-disc and cardiac structure. To address this question, a unique three-dimensional virtual muscle model was applied to gene expression data from transgenic mice with increased or decreased PI3K(p110a) activity under basal conditions (sham) and in a setting of myocardial infarction to display the location of structural proteins. Key findings from this analysis were then validated experimentally. The three-dimensional virtual muscle model visually highlighted reciprocally regulated transcripts associated with PI3K activation that encoded key components of the Z-disc and costamere, including melusin. Studies were performed to assess whether PI3K and melusin interact in the heart. Here, we identify a novel melusin- PI3K interaction that generates lipid kinase activity. The direct impact of PI3K(p110a) on myocyte structure was assessed by treating neonatal rat ventricular myocytes with PI3K(p110a) inhibitors and examining the myofiber morphology of hearts from PI3K transgenic mice. Results demonstrate that PI3K is critical for myofiber maturation and Z-disc alignment. In summary, PI3K regulates the expression of genes essential for cardiac structure and Z-disc signaling, interacts with melusin, and is critical for Z-disc alignment.
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    Journal Title
    Journal of Biological Chemistry
    Volume
    286
    Issue
    35
    DOI
    https://doi.org/10.1074/jbc.M111.271684
    Subject
    Gene Expression (incl. Microarray and other genome-wide approaches)
    Chemical Sciences
    Biological Sciences
    Medical and Health Sciences
    Publication URI
    http://hdl.handle.net/10072/44474
    Collection
    • Journal articles

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