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dc.contributor.authorJ. Cannata, Daviden_US
dc.contributor.authorJ. Crossley, Kellyen_US
dc.contributor.authorBarclay, Chrisen_US
dc.contributor.authorW. Walker, Daviden_US
dc.contributor.authorM. West, Janen_US
dc.date.accessioned2017-05-03T11:39:58Z
dc.date.available2017-05-03T11:39:58Z
dc.date.issued2011en_US
dc.date.modified2012-08-07T23:35:26Z
dc.identifier.issn1664042Xen_US
dc.identifier.doi10.3389/fphys.2011.00109en_US
dc.identifier.urihttp://hdl.handle.net/10072/44985
dc.description.abstractThe transition from fetal to postnatal life involves clearance of liquid from the lung and airways, and rapid formation of a functional residual capacity. Despite the importance of the diaphragm in this process, the impact of birth on the mechanical and functional activity of its muscle fibers is not known. This study determined the contractile characteristics of individual "skinned" diaphragm fibers from 70 days (0.47) gestation to after birth in sheep. Based on differential sensitivity to the divalent ions calcium (Ca2+) and strontium (Sr2+), all fibers in the fetal diaphragm were classified as "fast," whereas fibers from the adult sheep diaphragm exhibited a "hybrid" phenotype where both "fast" and "slow" characteristics were present within each single fiber. Transition to the hybrid phenotype occurred at birth, was evident after only 40 min of spontaneous breathing, and could be induced by simple mechanical stretch of diaphragm fibers from near-term fetuses (~147 days gestation). Both physical stretch of isolated fibers, and mechanical ventilation of the fetal diaphragm in situ, significantly increased sensitivity to Ca2+ and Sr2+, maximum force generating capacity, and decreased passive tension in near-term and preterm fetuses; however, only fibers from near-term fetuses showed a complete transition to a "hybrid" activation profile. These findings suggest that stretch associated with the transition from a liquid to air-filled lung at birth induces physical changes of proteins determining the activation and elastic properties of the diaphragm. These changes may allow the diaphragm to meet the increased mechanical demands of breathing immediately after birth.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.format.extent2070053 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglishen_US
dc.language.isoen_US
dc.publisherFrontiers Research Foundationen_US
dc.publisher.placeSwitzerlanden_US
dc.relation.ispartofstudentpublicationNen_US
dc.relation.ispartofpagefrom109-1en_US
dc.relation.ispartofpageto109-13en_US
dc.relation.ispartofjournalFrontiers in Physiologyen_US
dc.relation.ispartofvolume2en_US
dc.rights.retentionYen_US
dc.subject.fieldofresearchCell Physiologyen_US
dc.subject.fieldofresearchcode111601en_US
dc.titleContribution of stretch to the change of activation properties of muscle fibers in the diaphragm at the transition from fetal to neonatal lifeen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.rights.copyrightCopyright remains with the authors 2011. The attached file is reproduced here in accordance with the copyright policy of the publisher. For information about this journal please refer to the journal’s website or contact the authors.en_US
gro.date.issued2011
gro.hasfulltextFull Text


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