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dc.contributor.authorGoire, Namraj
dc.contributor.authorOhnishi, Makoto
dc.contributor.authorLimnios, Athena E
dc.contributor.authorLahra, Monica M
dc.contributor.authorLambert, Stephen B
dc.contributor.authorNimmo, Graeme R
dc.contributor.authorNissen, Michael D
dc.contributor.authorSloots, Theo P
dc.contributor.authorWhiley, David M
dc.date.accessioned2017-05-03T14:57:41Z
dc.date.available2017-05-03T14:57:41Z
dc.date.issued2012
dc.date.modified2014-08-28T22:15:51Z
dc.identifier.issn0305-7453
dc.identifier.doi10.1093/jac/dkr549
dc.identifier.urihttp://hdl.handle.net/10072/45629
dc.description.abstractObjectives Recent emergence of the extensively drug-resistant Neisseria gonorrhoeae H041 strain in Japan raises concerns that gonorrhoea may soon become untreatable and emphasizes the need for enhanced surveillance. In this study we developed a real-time PCR assay for direct detection of the H041 strain. Methods Two real-time PCR assays for detection of the penA gene of the H041 strain, H041-PCR1 and H041-PCR2, were developed and evaluated in parallel. Assay performance was assessed using a panel of pathogenic and commensal Neisseria species (n?=?167 strains) including the N. gonorrhoeae H041 strain and clinical specimens (n?=?252) submitted for sexual health screening. The detection limits of the assays were compared with a standard N. gonorrhoeae real-time PCR method. Results Both the H041-PCR1 and H041-PCR2 assays correctly detected the N. gonorrhoeae H041 strain and provided negative results for all other N. gonorrhoeae strains. However, only the H041-PCR2 assay proved to be specific when applied to the non-gonococcal Neisseria species and clinical samples. False-positive results in the H041-PCR1 included cross-reactions with two Neisseria subflava isolates and eight clinical specimens. DNA sequencing of these N. subflava strains revealed the presence of the penicillin-binding protein 2 Ala328Thr alteration previously only observed in the N. gonorrhoeae H041 strain. Conclusions The H041-PCR2 assay is suitable for direct detection of the N. gonorrhoeae H041 ceftriaxone-resistant strain in cultured and non-cultured samples.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherOxford University Press
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom902
dc.relation.ispartofpageto905
dc.relation.ispartofissue4
dc.relation.ispartofjournalJournal of Antimicrobial Chemotherapy
dc.relation.ispartofvolume67
dc.rights.retentionY
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchPharmacology and pharmaceutical sciences
dc.subject.fieldofresearchcode3107
dc.subject.fieldofresearchcode3207
dc.subject.fieldofresearchcode3214
dc.titleEnhanced gonococcal antimicrobial surveillance in the era of ceftriaxone resistance: a real-time PCR assay for direct detection of the Neisseria gonorrhoeae H041 strain
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2012
gro.hasfulltextNo Full Text
gro.griffith.authorNimmo, Graeme R.


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