dc.contributor.author | Goire, Namraj | |
dc.contributor.author | Ohnishi, Makoto | |
dc.contributor.author | Limnios, Athena E | |
dc.contributor.author | Lahra, Monica M | |
dc.contributor.author | Lambert, Stephen B | |
dc.contributor.author | Nimmo, Graeme R | |
dc.contributor.author | Nissen, Michael D | |
dc.contributor.author | Sloots, Theo P | |
dc.contributor.author | Whiley, David M | |
dc.date.accessioned | 2017-05-03T14:57:41Z | |
dc.date.available | 2017-05-03T14:57:41Z | |
dc.date.issued | 2012 | |
dc.date.modified | 2014-08-28T22:15:51Z | |
dc.identifier.issn | 0305-7453 | |
dc.identifier.doi | 10.1093/jac/dkr549 | |
dc.identifier.uri | http://hdl.handle.net/10072/45629 | |
dc.description.abstract | Objectives Recent emergence of the extensively drug-resistant Neisseria gonorrhoeae H041 strain in Japan raises concerns that gonorrhoea may soon become untreatable and emphasizes the need for enhanced surveillance. In this study we developed a real-time PCR assay for direct detection of the H041 strain. Methods Two real-time PCR assays for detection of the penA gene of the H041 strain, H041-PCR1 and H041-PCR2, were developed and evaluated in parallel. Assay performance was assessed using a panel of pathogenic and commensal Neisseria species (n?=?167 strains) including the N. gonorrhoeae H041 strain and clinical specimens (n?=?252) submitted for sexual health screening. The detection limits of the assays were compared with a standard N. gonorrhoeae real-time PCR method. Results Both the H041-PCR1 and H041-PCR2 assays correctly detected the N. gonorrhoeae H041 strain and provided negative results for all other N. gonorrhoeae strains. However, only the H041-PCR2 assay proved to be specific when applied to the non-gonococcal Neisseria species and clinical samples. False-positive results in the H041-PCR1 included cross-reactions with two Neisseria subflava isolates and eight clinical specimens. DNA sequencing of these N. subflava strains revealed the presence of the penicillin-binding protein 2 Ala328Thr alteration previously only observed in the N. gonorrhoeae H041 strain. Conclusions The H041-PCR2 assay is suitable for direct detection of the N. gonorrhoeae H041 ceftriaxone-resistant strain in cultured and non-cultured samples. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Oxford University Press | |
dc.publisher.place | United Kingdom | |
dc.relation.ispartofstudentpublication | N | |
dc.relation.ispartofpagefrom | 902 | |
dc.relation.ispartofpageto | 905 | |
dc.relation.ispartofissue | 4 | |
dc.relation.ispartofjournal | Journal of Antimicrobial Chemotherapy | |
dc.relation.ispartofvolume | 67 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Microbiology | |
dc.subject.fieldofresearch | Medical microbiology | |
dc.subject.fieldofresearch | Pharmacology and pharmaceutical sciences | |
dc.subject.fieldofresearchcode | 3107 | |
dc.subject.fieldofresearchcode | 3207 | |
dc.subject.fieldofresearchcode | 3214 | |
dc.title | Enhanced gonococcal antimicrobial surveillance in the era of ceftriaxone resistance: a real-time PCR assay for direct detection of the Neisseria gonorrhoeae H041 strain | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.date.issued | 2012 | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Nimmo, Graeme R. | |