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dc.contributor.authorS. Bradbury, Michelleen_US
dc.contributor.authorPanagiotakos, Georgiaen_US
dc.contributor.authorK. Chan, Billen_US
dc.contributor.authorTomishima, Marken_US
dc.contributor.authorZanzonico, Paten_US
dc.contributor.authorVider, Jelenaen_US
dc.contributor.authorPonomarev, Vladimiren_US
dc.contributor.authorStuder, Lorenzen_US
dc.contributor.authorTabar, Vivianeen_US
dc.description.abstractPre-clinical efforts of grafting human embryonic stem cell (hESC)-derived neural precursors have been hampered by problems ranging from graft rejection to overgrowth and tumor formation. The ability to detect such potential complications sensitively and reliably in clinically relevant contexts will rest upon the implementation of suitable non-invasive imaging technologies for continuously probing graft survival, proliferation, and migration. Neural precursors were transduced ex vivo using a lentiviral-mediated gene delivery system expressing firefly D-luciferase, under the control of a cytomegalovirus promoter. Transduced cells revealed no loss of cellular morphology, proliferative capacity, or neural phenotype in vitro. As a novel approach to monitoring the fate of human grafts within the living brain, we adapted optical bioluminescence imaging to assess long-term graft viability in immunodeficient mouse models transplanted with genetically engineered human neural precursor cells. We additionally applied this technology to immunocompetent models for detecting and characterizing the time course of graft rejection. Using this strategy, we define statistically relevant imaging criteria that can predict graft rejection or overgrowth. In conclusion, our data suggest that optical bioluminescence imaging can serve as an essential tool for the development of hESC-based grafting strategies in the CNS.en_US
dc.publisherWiley-Blackwell Publishing Ltd.en_US
dc.publisher.placeUnited Kingdomen_US
dc.relation.ispartofjournalJournal of Neurochemistryen_US
dc.subject.fieldofresearchNeurosciences not elsewhere classifieden_US
dc.titleOptical bioluminescence imaging of human ES cell progeny in the rodent CNSen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.hasfulltextNo Full Text

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