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  • Expression, purification, crystallization and preliminary X-ray diffraction analysis of a ribokinase from the thermohalophile Halothermothrix orenii

    Author(s)
    Kori, Lokesh D
    Hofmann, Andreas
    Patel, Bharat KC
    Griffith University Author(s)
    Patel, Bharat K.
    Hofmann, Andreas
    Kori, Lokesh
    Year published
    2012
    Metadata
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    Abstract
    A ribokinase gene (rbk) from the anaerobic halothermophilic bacterium Halothermothrix orenii was cloned and overexpressed in Escherichia coli. The recombinant protein (Ho-Rbk) was purified using immobilized metal-ion affinity chromatography and crystals were obtained using the sitting-drop method. Diffraction data were collected to a resolution of 3.1 A ࠵sing synchrotron radiation. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 45.6, b = 61.1, c = 220.2, and contained two molecules per asymmetric unit. A molecular-replacement solution has been found and attempts are ...
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    A ribokinase gene (rbk) from the anaerobic halothermophilic bacterium Halothermothrix orenii was cloned and overexpressed in Escherichia coli. The recombinant protein (Ho-Rbk) was purified using immobilized metal-ion affinity chromatography and crystals were obtained using the sitting-drop method. Diffraction data were collected to a resolution of 3.1 A ࠵sing synchrotron radiation. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 45.6, b = 61.1, c = 220.2, and contained two molecules per asymmetric unit. A molecular-replacement solution has been found and attempts are currently under way to build a model of the ribokinase. Efforts to improve crystal quality so that higher resolution data can be obtained are also being considered.
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    Journal Title
    Acta Crystallographica Section F
    Volume
    68
    DOI
    https://doi.org/10.1107/S1744309111041091
    Subject
    Structural biology (incl. macromolecular modelling)
    Publication URI
    http://hdl.handle.net/10072/45882
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    • Journal articles

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