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  • Loss of adenosine A2B receptor mediated relaxant responses in the aged female rat bladder; effects of dietary phytoestrogens

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    Author(s)
    Owen, Suzzanne J
    Massa, Helen H
    Rose'Meyer, Roselyn B
    Griffith University Author(s)
    Massa, Helen M.
    Owen, Suzzanne J.
    Rose'Meyer, Roselyn B.
    Year published
    2012
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    Abstract
    This study examined the effect of age, ovariectomy and dietary phytoestrogen ingestion on adenosine A2B receptor mediated relaxant responses and mRNA expression of adenosine receptor subtypes in the rat isolated bladder. Female Wistar rats (8 weeks) were anaesthetised and the ovaries were removed (ovx) or left intact (sham). Rats were fed either normal rat chow (soy, phytoestrogens) or a non-soy (phytoestrogen free) diet. Isolated bladder from rats aged 12, 24 or 52 weeks were pre-contracted with 3 占carbachol prior to a concentration response curve to 5'-(N-ethylcarboxamido) adenosine (NECA) being obtained. In ...
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    This study examined the effect of age, ovariectomy and dietary phytoestrogen ingestion on adenosine A2B receptor mediated relaxant responses and mRNA expression of adenosine receptor subtypes in the rat isolated bladder. Female Wistar rats (8 weeks) were anaesthetised and the ovaries were removed (ovx) or left intact (sham). Rats were fed either normal rat chow (soy, phytoestrogens) or a non-soy (phytoestrogen free) diet. Isolated bladder from rats aged 12, 24 or 52 weeks were pre-contracted with 3 占carbachol prior to a concentration response curve to 5'-(N-ethylcarboxamido) adenosine (NECA) being obtained. In 12-week-old rats, the bladder exhibited enhanced relaxant responses to NECA in soy-fed rats (P<0.05), whilst at 24 weeks of age, the relaxant responses to NECA were attenuated in all the groups studied except soy-treated sham rat bladders in which the relaxant responses were enhanced. At 52 weeks of age, no relaxant effects were observed in any of the treatment groups and NECA-induced contractile responses occurred. In all bladders, the adenosine A2B receptor was the most abundantly expressed. In bladders from young and mature female rats, the mRNA expression of adenosine receptors (A1, A2A and A2B) was lowest in the bladder from non-soy-fed ovariectomised animals and the use of phytoestrogens in the diet increased the mRNA expression of these receptors (P<0.05).While a soy diet improves the relaxant effects to the adenosine analogue via adenosine A2B receptors in bladders from younger rats, the benefits are lost with advancing age.
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    Journal Title
    Naunyn-Schmiedeberg Archives of Pharmacology
    Volume
    385
    Issue
    5
    DOI
    https://doi.org/10.1007/s00210-011-0722-y
    Copyright Statement
    © 2012 Springer-Verlag. This is an electronic version of an article published in Naunyn-Schmiedeberg's Archives of Pharmacology, May 2012, Volume 385, Issue 5, pp 539-549. Naunyn-Schmiedeberg's Archives of Pharmacology is available online at: http://link.springer.com// with the open URL of your article.
    Subject
    Basic Pharmacology
    Pharmacology and Pharmaceutical Sciences
    Medical Physiology
    Publication URI
    http://hdl.handle.net/10072/46078
    Collection
    • Journal articles

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