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dc.contributor.authorKotiw, Michaelen_US
dc.contributor.authorJohnson, Meganen_US
dc.contributor.authorPandey, Manishaen_US
dc.contributor.authorFry, Scotten_US
dc.contributor.authorL. Hazell, Stuarten_US
dc.contributor.authorJ. Netter, Hansen_US
dc.contributor.authorGood, Michaelen_US
dc.contributor.authorOlive, Colleenen_US
dc.description.abstractVirus-like particles (VLPs) based on the small envelope protein of hepatitis B virus (HBsAg-S) are immunogenic at the B- and T-cell level. In this study, we inserted overlapping sequences encoding the carboxy terminus of the Helicobacter pylori katA gene product into HBsAg-S. The HBsAg-S-KatA fusion proteins were able to assemble into secretion-competent VLPs (VLP-KatA). The VLP-KatA proteins were able to induce KatA-specific antibodies in immunized mice. The mean total IgG antibody titers 41 days post-primary immunization with VLP-KatA (2.3 נ103) were significantly greater (P < 0.05) than those observed for vaccination with VLP alone (5.2 נ102). Measurement of IgG isotypes revealed responses to both IgG1 and IgG2a (mean titers, 9.0 נ104 and 2.6 נ104, respectively), with the IgG2a response to vaccination with VLP-KatA being significantly higher than that for mice immunized with KatA alone (P < 0.05). Following challenge of mice with H. pylori, a significantly reduced bacterial load in the gastric mucosa was observed (P < 0.05). This is the first report describing the use of VLPs as a delivery vehicle for H. pylori antigens.en_US
dc.publisherAmerican Society for Microbiologyen_US
dc.publisher.placeUnited Statesen_US
dc.relation.ispartofjournalClinical and Vaccine Immunologyen_US
dc.subject.fieldofresearchInfectious Agentsen_US
dc.titleImmunological Response to Parenteral Vaccination with Recombinant Hepatitis B Virus Surface Antigen Virus-Like Particles Expressing Helicobacter pylori KatA Epitopes in a Murine H. pylori Challenge Modelen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
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